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Application of DUSP12 in hepatic ischemia reperfusion injury (IRI)

A 1.DUSP12, reperfusion injury technology, applied in the field of biomedicine, can solve the problem that the pathophysiological effect has not been fully confirmed, and achieve the effect of reducing liver ischemia-reperfusion injury

Inactive Publication Date: 2021-04-20
WUHAN UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, the pathophysiological role of DUSP12 in liver ischemia-reperfusion has not been well established

Method used

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  • Application of DUSP12 in hepatic ischemia reperfusion injury (IRI)
  • Application of DUSP12 in hepatic ischemia reperfusion injury (IRI)
  • Application of DUSP12 in hepatic ischemia reperfusion injury (IRI)

Examples

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Embodiment Construction

[0078] Below in conjunction with specific embodiment. To further set forth the present invention, it should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention. The specific experimental conditions and methods not indicated in the following examples are usually according to conventional conditions such as: U.S. National The conditions described in the Guide for the Care and Use of Laboratory Animals of the Institute of Health; D.L. Spector et al., Science Press, 2001, Guidelines for Cell Experiments, etc., or as recommended by the manufacturer.

[0079] 1. Animal Models and Procedures

[0080] The generation of hepatocyte-specific DUSP12 knockout mice (CKO) and hepatocyte-specific DUSP12 transgenic mice (TG) was similar to previous experiments. CKO pups (Flox) and TG pups (NTG) were used as controls. The experiments were carried out in the Animal Experiment Center o...

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Abstract

The invention discloses application of DUSP12 in hepatic ischemia reperfusion injury (IRI). A hepatocyte specific DUSP12 gene knockout mouse and a hepatocyte specific DUSP12 transgenic mouse are selected as test objects, mouse hepatic IRI models are constructed respectively, then isolated culture of primary hepatocytes and construction of hepatocyte in-vitro anoxia / reoxygenation are performed, and the function of DUSP12 in IRI is studied; and the new function of the DUSP12 gene in hepatic IRI is discovered, and DUSP12 overexpression can relieve hepatic IRI by inhibiting inflammation and cell apoptosis, so that the DUSP12 can be applied to screening or preparation of a drug for preventing / treating hepatic IRI, and a new drug target is provided for hepatic IRI.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of DUSP12 in liver ischemia-reperfusion injury. Background technique [0002] Hepatic ischemia-reperfusion injury (IRI) is a common physiological phenomenon in trauma, shock and liver surgery. Severe IRI injury caused by prolonged warm ischemia often leads to liver function damage or even irreversible liver failure. Many drugs and genetic engineering methods are designed to improve ischemia-reperfusion injury, however, there are no clinically feasible drugs and methods to prevent and treat liver ischemia-reperfusion injury. More and more studies have shown that the initiation of liver inflammatory response is a key step in liver ischemia-reperfusion injury. During liver ischemia, hypoxia and glycogen depletion lead to hepatocellular injury. Hepatocytes produce reactive oxygen species under ischemia stimulation, which leads to apoptosis of hepatocytes after ...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P1/16A61P9/10
Inventor 邱涛周江桥王天宇陈忠宝马枭雄张龙邹寄林
Owner WUHAN UNIV
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