Electrode array device for gene sequence synthesis and control method thereof

A gene sequence and electrode array technology, which is applied in the field of electrode array devices, can solve the problems of complex processing technology, high electrode cost, complicated procedures and the like, and achieve the effects of simple device structure, convenient preparation and simple control.

Active Publication Date: 2021-04-16
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to this method of gene synthesis, it is necessary to use micro-processing technology to make micro-metal electrodes on the surface of the silicon substrate. Although the electrodes can be made very small, the cost of making microarray electrodes on the surface of the silicon substrate is relatively high, and the processing technology is complicated. And it is necessary to use the bonding process to connect the microelectrode array to the peripheral circuit control system, the process is more complicated and difficult to control

Method used

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  • Electrode array device for gene sequence synthesis and control method thereof
  • Electrode array device for gene sequence synthesis and control method thereof
  • Electrode array device for gene sequence synthesis and control method thereof

Examples

Experimental program
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Embodiment Construction

[0025] The preparation method of the electrode array substrate gene synthesis electrode array uses the metal deposition process to form an electrode array unit on the surface of the FR-4 epoxy glass cloth laminate. By applying a certain voltage to the array unit, the gene sequence is controlled in the array unit. on the synthesis.

[0026] The metal material of the array electrodes is one of Au, Pt and Ir.

[0027] By controlling the on-off or voltage of high and low levels, the potential difference between the anode and cathode on any array unit can be precisely controlled, thereby controlling the specific site of gene synthesis.

[0028] Schematic diagram of electrode-controlled gene synthesis figure 2 shown, including:

[0029] (1) Control the on-off or voltage of the high and low levels of the anode and the cathode. The "-" below the cathode in the figure indicates that a low level or a smaller voltage is applied at the cathode. The "+" below the anode indicates that a ...

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Abstract

The invention belongs to the technical field of electronic information and biotechnology crossing, and particularly relates to an electrode array device for gene sequence synthesis and a control method of the electrode array device. The electrode array device of the present invention comprises a substrate and an electrode array formed on the substrate, an electrode unit comprises an anode and a cathode, and the substrate is covered with a microfluidic cover; and the device further comprises a microfluidic module and a control module, and the control module accurately controls the potential difference between the anode and the cathode on an array unit by controlling the on-off of high and low levels of the output voltage or the magnitude of the voltage; and the microfluidic module is controlled to accurately control the transportation of various reagents required by gene synthesis. The device disclosed by the invention is simple in structure and convenient to prepare, and does not need a complex micromachining process; and when the device is used for gene sequence synthesis, control is simple, and industrialization and large-scale application are facilitated.

Description

technical field [0001] The invention belongs to the technical field intersecting electronic information and biotechnology, and in particular relates to an electrode array device for gene sequence synthesis and a control method thereof. Background technique [0002] DNA synthesis plays an important role in life sciences, such as identifying genetic mutations, analyzing data generated by large-scale genome sequencing, and analyzing DNA-protein interactions. At the same time, DNA synthesis technology will play a vital role in the field of information storage in the future. DNA storage technology, with its advantages of small size, high data density, and strong stability, will become an important technical path to solve the series of crises caused by the data explosion in the future. The principle of DNA storage is to convert digital signals into chemical signals. When encoding and storing, it is a synthetic base DNA sequence, and when it is decoded and read, it is a sequenced ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 孙鹏吴东平徐刚伟杨力建
Owner FUDAN UNIV
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