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Construction method of prostate cancer radiation tolerance cell strain

A technology for radiation tolerance and prostate cancer, which is applied in the field of radiation biology and can solve the problem of lack of radiation-resistant cell lines for prostate cancer

Inactive Publication Date: 2021-04-16
LANZHOU UNIVERSITY
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Problems solved by technology

[0006] In order to overcome the problem of lack of radiation-resistant prostate cancer cell lines, the present invention provides a method for constructing radiation-resistant prostate cancer cell lines with stable radiation resistance

Method used

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  • Construction method of prostate cancer radiation tolerance cell strain

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Embodiment 1

[0017] A method for establishing a radiation-resistant cell line of human prostate cancer, the method comprising:

[0018] 1. Cell culture: Human prostate cancer cell line DU145 was cultured in DMEM medium containing 10% fetal bovine serum after resuscitation, cultured at 37°C and 5% CO2, and passaged once every 3 days.

[0019] 2. Irradiation conditions: Take DU145 cells in logarithmic growth phase and seal the culture dish with parafilm to avoid cell contamination. The cells were irradiated under the X-ray apparatus, the irradiation energy was 225ekV, the irradiation distance was 60cm, and the dose rate was in the range of 101.5-112.4Gy / min.

[0020] 3. Irradiation method: Take the human prostate cancer cell line DU145 in the exponential growth phase, according to 5×10 per dish 5 Cells were seeded in 60 mm Petri dishes. X-ray irradiation was performed when the cells grew to 60%, and the irradiation dose was 4Gy. Immediately after irradiation, the medium was replaced with ...

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Abstract

The invention discloses a construction method and application of a human prostate cancer radiation tolerance cell strain. The method comprises the following steps: taking a human prostate cancer cell strain DU145 in an exponential growth phase, and inoculating the human prostate cancer cell strain DU145 into a 60mm culture dish according to the density of 5 * 10<5> cells per dish; when the cells grow to 60%, carrying out X-ray irradiation, wherein the irradiation dose is 4Gy; continuously culturing the cells until the density reaches 90%, digesting with pancreatin, counting, and inoculating into a 60mm culture dish according to the density of 5 * 10 <5> cells per dish; repeating the steps of irradiation and culture, and continuing stable passage for 30 days when the accumulated irradiation dose of the cells reaches 60Gy; and after the human prostate cancer radiation-tolerant cell strain which grows stably, is subjected to passage and is resuscitated is obtained, cryopreservingthe cells in a liquid nitrogen tank in time. Small-dose X-rays are selected for cell irradiation, and the method is characterized in that the clinical radiation treatment process is simulated, the irradiation dose is small, the cell survival rate is high, and the cell drug resistance is stable.

Description

technical field [0001] The invention belongs to the field of radiation biology, in particular to a method for constructing prostate cancer radiation-resistant cell lines. Background technique [0002] Prostate cancer refers to epithelial malignant tumors that occur in the prostate. It is the second most common cancer in men worldwide and one of the leading causes of death in male cancer patients (Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin:2018,68:394-424.). The World Health Organization's cancer report shows that in 2018, there were 1.44 million new prostate cancer patients worldwide, accounting for 14.3% of the total number of new cancers; 375,000 deaths, accounting for 6.8% of global cancer deaths. Therefore, prostate cancer should be focused on in future cancer prevention and control. [0003] At present, the treatment methods for p...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12N13/00A01N1/02
Inventor 郭梦环张红甘露
Owner LANZHOU UNIVERSITY
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