Method for generating and inducing pluripotent stem cells by using pig fibroblasts
A technology of pluripotent stem cells and pig fibroblasts, applied in the field of pluripotent stem cells, can solve the problems of difficulty in feeding, development into tumors, long cycle, etc., and achieve the effect of optimizing iPS induction strategy and easy gene manipulation
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[0029] In order to make the present invention easier to understand, the present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.
[0030] The practice of the present invention will employ, unless otherwise indicated, conventional techniques of molecular biology, microbiology, cell biology, immunology and recombinant DNA, which are within the skill of the art. See, e.g., Sambrook, Fritsch, and Maniatis, A Laboratory Guide to Molecular Cloning, 3rd Edition (2002); CURRENT PROTOCOLSIN MOLECULAR BIOLOGY (eds. F.M. Ausubel et al. (1987)); SERIES METHODS INENZYMOLOGY (Academic Press, Inc.): PCR 2: A PRACTICAL APPROACH (Eds. M.J. MacPherson, B.D.Hames and G.R. Taylor, (1995)), Harlow and Lane, eds. (1988) ANTIBODIES, ALABORATORY MANUAL and ANIMAL CELLCULTURE (Eds. R.I. Freshney, (1987)); W. Fren...
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