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Pair of molecular marker primers closely linked with corncob coarse QTL ZmED3 and application thereof

A molecular marker, corn ear technology, applied in the biological field, can solve problems such as lack

Active Publication Date: 2021-04-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there are few related studies on QTLs controlling corn ear diameter traits, and there is still a lack of a technical solution that can accurately identify the traits that affect corn ear diameter

Method used

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  • Pair of molecular marker primers closely linked with corncob coarse QTL ZmED3 and application thereof
  • Pair of molecular marker primers closely linked with corncob coarse QTL ZmED3 and application thereof
  • Pair of molecular marker primers closely linked with corncob coarse QTL ZmED3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] Development of Molecular Markers Tightly Linked to Corn Ear Thickness Traits

[0044] 1 Materials and methods

[0045] 1.1 Test material

[0046] ZmED3 near-isogenic lines Ye478 and Ye478 ed3 and the progeny mapping population constructed by near-isogenic lines;

[0047] Natural population materials composed of 513 maize inbred lines with wide sources and rich genetic variation.

[0048] 1.2 Test method

[0049] 1.2.1 Field test and phenotypic identification

[0050] For the heterozygous recombinant single plant used for the offspring test, each heterozygous single plant develops into no less than 200 offspring families. Homozygous recombination families of 6 different exchange types were planted in winter in Sanya, Hainan (18°N, 109°E) in 2017. Random blocks were adopted, with 3 repetitions. The row length was 3m, the width was 0.6m, and 12 trees were planted in each row. After maturity, the ears were harvested, air-dried, and abnormal ears were removed, and the ...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a pair of molecular marker primers closely linked with corncob coarse QTL ZmED3 and application thereof. The invention provides the pair of molecular marker primers closely linked with the corncob coarse QTL ZmED3, an upstream primer of the molecular marker primers is shown as SEQ ID NO:1, and a downstream primer of the molecular marker primers is shown as SEQ ID NO:2. The molecular marker primers have a close linkage relationship with a gene for controlling the coarse character of corncobs, can be used for accurately amplifying to obtain a nucleotide sequence shown as SEQ ID NO:3 or SEQ ID NO:4, and have the characteristic of high amplification specificity. The molecular marker primers can effectively achieve the technical effect of identifying the corncob thick character, and can be effectively applied to genetic improvement of the corncob thick character.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pair of molecular marker primers closely linked with corn ear coarse QTL ZmED3 and application thereof. Background technique [0002] Corn (Zea may L.) is the most important food, feed, industrial raw material and energy crop in the world today, and it plays a huge role in ensuring world food security, economic development and alleviating energy crisis. Due to the rigid constraints of land supply, in order to meet the demand for corn in the sustainable development of my country's national economy, increasing the yield of corn per unit area is a major issue in genetics and breeding. [0003] Enhancing the selection of maize ear diameter traits is a feasible way to increase yield per plant. Searching for molecular markers closely linked to yield QTLs, cloning key genes and analyzing their genetic regulation pathways have long been the research hotspots of molecular breede...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 邱法展孙琴龚佃明肖姿仪王苑如
Owner HUAZHONG AGRI UNIV
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