Experimental method for analyzing influence of apis cerana odorant receptor AcerOr2 on semen quality

A Chinese honeybee, odorant receptor technology, applied in analytical materials, biomaterial analysis, biochemical equipment and methods, etc., can solve problems affecting bee semen quality and other issues

Pending Publication Date: 2021-04-06
SHANXI AGRI UNIV
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  • Experimental method for analyzing influence of apis cerana odorant receptor AcerOr2 on semen quality
  • Experimental method for analyzing influence of apis cerana odorant receptor AcerOr2 on semen quality
  • Experimental method for analyzing influence of apis cerana odorant receptor AcerOr2 on semen quality

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[0027] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] see figure 1 , the present invention provides a kind of technical scheme: the experimental method of the influence analysis of Chinese bee odorant receptor AcerOr2 on semen quality, comprises the following steps:

[0029] S1. First prepare the following reagents and instruments:

[0030] Reagents include: rabbit anti-CaM, rabbit anti-CaMKII, rabbit anti-p-CaMKII were purchased from Bioworld Technology; VUAA1 and W7 were purchased from Sigma; Annexin V PE / 7-AAD cell apoptosis kit was purchased from Kangwei Century; TRIzolTM was purchased from Invitrogen Company; SYBR Premix Ex TaqTMⅡ, Prime...

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Abstract

The invention is applicable to the technical field of honeybee odorant receptor research, and provides an experimental method for analyzing the influence of an apis cerana odorant receptor AcerOr2 on semen quality, which comprises the following steps of carrying out fluorescent quantitative detection on the expression of a target gene, carrying out Western blot detection on the expression of a target gene protein, and evaluating conventional indexes of sperms by using a CASA computer-assisted analyzer, finally, detecting the integrity of sperm DNA through a flow cytometer, so that the expression of the odorant receptor AcerOr2 and related data of the influence of the odorant receptor AcerOr2 on the semen quality of the bees are obtained, and the possible biological effects of the apis cerana AcerOr2 and downstream signal molecules thereof in regulating the semen quality at the cellular level and the influence of the apis cerana AcerOr2 on the semen quality can be discussed, and a theoretical basis is provided for functional research of the odorant receptor in the reproductive process.

Description

technical field [0001] The invention belongs to the technical field of honeybee odorant receptor research, in particular to an experimental method for analyzing the influence of the Chinese honeybee odorant AcerOr2 on the quality of semen. Background technique [0002] Insect olfactory receptors (ORs) are seven transmembrane ion receptors. They are predominantly expressed on the dendritic membranes of olfactory and gustatory organ neurons (ORNs) and are involved in detecting volatile odors and converting these chemical signals into electrical signals. Insect olfactory neurons express two classes of odorant receptors: traditional odorant receptors (ORs) and odorant co-receptors (Orco). The OR / Orco complex forms a functional cation channel that allows the K + and Ca 2+ Enters sensory cells after odorant receptors bind to an odor. A conserved binding region for calmodulin (CaM) was found in the intracellular loop 2 domain of Orco, suggesting that CaM activity may affect the...

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Application Information

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IPC IPC(8): C12Q1/6851G01N33/68
CPCC12Q1/6851G01N33/68G01N2333/705C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 郭丽娜杨姗姗赵慧婷马卫华杨爽姜玉锁
Owner SHANXI AGRI UNIV
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