Full-automatic nucleic acid methylation amplification detection equipment

Abstract

The invention discloses full-automatic nucleic acid methylation amplification detection equipment. The equipment comprises a control mechanism, a pre-treatment mechanism, a post-treatment mechanism, a transfer mechanism and a detector; the control mechanism is communicated with the pre-treatment mechanism, the post-treatment mechanism and the transfer mechanism; the pre-treatment mechanism is communicated with the post-treatment mechanism through the transfer mechanism; the pre-treatment mechanism comprises a first magnetic bar sleeve, a first deep hole plate, a first oscillation assembly and a first magnetic attraction assembly; the first deep hole plate is used for containing a to-be-treated liquid; the first oscillation assembly is connected with the first deep hole plate; the first oscillation assembly is located below the first deep hole plate and used for oscillating liquid in the first deep hole plate; the first magnetic attraction assembly is used for primarily separating a to-be-treated detection sample in the to-be-treated liquid; and the post-treatment mechanism comprises a second deep hole plate, a second oscillation assembly and a second magnetic attraction assembly and is used for realizing sulfite conversion and purification of DNA.

Description

technical field [0001] The present application relates to the technical field of molecular diagnosis, in particular to a fully automatic nucleic acid methylation amplification detection device. Background technique [0002] DNA methylation is an important research content of epigenetics. The change of DNA methylation status is an important factor that causes tumorigenesis. This change includes the decrease of the overall methylation level of the genome and the abnormal increase of the local methylation level of CpG islands, which leads to the instability of the genome and induces cell carcinogenesis. [0003] Variations at the epigenetic level are more frequent than gene mutations, so the use of epigenetic variation to diagnose tumors has higher sensitivity and better specificity than detection at the gene mutation level. Because DNA methylation is an early event in tumorigenesis, early diagnosis of tumors by detecting the methylation level of genes has great clinical appli...

AI Technical Summary

Problems solved by technology

And the manual time is long, the steps are numerous and complicated, and the operation pressure of more than 32 samples is greatly increased. The numerous intermediate steps make the stagnation time of the front and back samples different, and the repeatability of the sample detection is not high.

One of the difficulties in developing automated equipment at present is the processing of large volume samples. The volume of the intermediate processing mixture can reach 10mL, and there are no corresponding consumables to match the automated equipment.

Images