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Ultraviolet sterilization synergist and method for sterilizing by combining ultraviolet sterilization synergist with ultraviolet rays

A synergist, ultraviolet technology, applied in the direction of fungicides, biocides, biocides, etc., can solve the problem of less sterilization synergy

Active Publication Date: 2021-03-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported synergistic effect of essential oils on ultraviolet sterilization is still relatively small, and it is urgent to add new ultraviolet antibacterial synergists to increase the bactericidal effect of ultraviolet rays on various occasions.

Method used

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  • Ultraviolet sterilization synergist and method for sterilizing by combining ultraviolet sterilization synergist with ultraviolet rays
  • Ultraviolet sterilization synergist and method for sterilizing by combining ultraviolet sterilization synergist with ultraviolet rays
  • Ultraviolet sterilization synergist and method for sterilizing by combining ultraviolet sterilization synergist with ultraviolet rays

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The bactericidal effect of embodiment 1 different ultraviolet irradiation time to ATCC 25922 bacterial strain

[0026] 1. Experimental materials:

[0027] (1) The ultraviolet lamp used in the test is PHILIPS TL-D brand; wattage: 18W; voltage: 220V; wavelength: (UVA) 300-469nm; tube diameter: 25mm; length: 60cm.

[0028] (2) Test medium: Cool the autoclaved MH agar medium and MacConkey agar medium (purchased from Guangdong Huankai Microbiology Technology Co., Ltd.) to 40°C, and use a pipette gun to take 20mL to sterile culture Dish and let it dry naturally for 30 minutes to prepare MH agar medium and MacConkey agar medium.

[0029] Escherichia coli standard strain ATCC25922 (preserved in the laboratory).

[0030] 2. Preparations before the test:

[0031] (1) Turn on the ultraviolet lamp and continue to irradiate for 30 minutes to preheat;

[0032] (2) The Escherichia coli standard strain ATCC25922 was cultured to a suitable size on MacConkey medium.

[0033] 3. Eval...

Embodiment 2

[0042] Example 2 Determination of the MIC of different concentrations of bergamot essential oils to ATCC 25922 bacterial strains

[0043] 1. Experimental materials:

[0044] (1) Test: The autoclaved MH broth was cooled for later use.

[0045] (2) Resin Azure: Macklin (Macklin) brand, MW is 251.17, and purity is 90%;

[0046] Escherichia coli standard strain ATCC25922 (preserved in the laboratory).

[0047] 2. Preparations before the test:

[0048] (1) Take the bergamot essential oil out of the refrigerator and equilibrate it to room temperature, absorb some of it for later use;

[0049] (2) The Escherichia coli standard strain ATCC 25922 was cultured to a suitable size on MacConkey agar medium.

[0050] (3) Weigh 0.2512 g of resin azure powder into a centrifuge tube, add 10 mL of pure water to dissolve, and the concentration of resin azure solution is 10 mM / L.

[0051] 3. Evaluation experiment of the bactericidal effect of bergamot essential oil:

[0052] (1) Inoculate a...

Embodiment 3

[0062] Example 3 The bactericidal effect of bergamot essential oil on ATCC 25922 bacterial strain after 30 minutes:

[0063] 1. Experimental materials:

[0064] (1) Experimental medium: cool the autoclaved MH agar medium and MacConkey agar medium (purchased from Guangdong Huankai Microbiology Technology Co., Ltd.) to 40°C, and use a pipette to take 20mL to sterile culture Dish and let it dry naturally for 30 minutes to prepare MH agar medium and MacConkey agar medium.

[0065] Escherichia coli standard strain ATCC25922 (preserved in the laboratory).

[0066] 2. Preparations before the test:

[0067] (1) Take the bergamot essential oil out of the refrigerator and equilibrate it to room temperature, absorb some of it for later use;

[0068] (2) Cultivate Escherichia coli standard strain ATCC 25922 on MacConkey medium to a suitable size.

[0069] 3. Evaluation experiment on bactericidal effect of bergamot essential oil:

[0070] (1) Inoculate a single colony of Escherichia c...

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Abstract

The invention belongs to the technical field of ultraviolet sterilization, and discloses an ultraviolet sterilization synergist and an ultraviolet combined sterilization method thereof. Based on the weak sterilization effect (the sterilization amount is 0.5-1.5 log when the bergamot essential oil acts alone) of A-waveband ultraviolet (UVA) and bergamot essential oil, the ultraviolet sterilizationsynergist has an obvious synergistic sterilization effect after combined action. Compared with a traditional ultraviolet sterilization technology, bacteria can be killed more efficiently, and the influence of the quality of the ultraviolet lamp on the disinfection effect is reduced.

Description

technical field [0001] The invention relates to the technical field of ultraviolet sterilization, more specifically, to an ultraviolet sterilization synergist and a method for sterilizing it in combination with ultraviolet rays. Background technique [0002] Ultraviolet germicidal technology is a convenient method with no chemical residues and low impact on the environment, and is often used to disinfect gases, liquids and solid surfaces. Ultraviolet light is a general term for radiation with a wavelength of 400nm-10nm in the electromagnetic spectrum, which cannot cause people's vision. It is invisible light with a higher frequency than blue-violet light. Ultraviolet rays are classified into UVA, UVB, UVC and UVD. Ultraviolet lamp sterilization is used in various places due to its low cost, convenient use, and no drug resistance. In recent years, due to the extensive use of antibiotics, bacterial drug resistance has become more and more serious. With the emergence of multi...

Claims

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Application Information

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IPC IPC(8): A01N65/36A01P3/00A01P1/00A61L2/10A61L2/18A61L101/56
CPCA01N65/00A01N65/36A61L2/10A61L2/18
Inventor 孙坚何慧灵崔泽华刘雅红廖晓萍
Owner SOUTH CHINA AGRI UNIV
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