Human mesenchymal stem cell-based extracellular matrix-derived composite collagen extracting solution hydrogel and preparation method thereof

Abstract

The embodiment of the invention discloses a human mesenchymal stem cell-based extracellular matrix-derived composite collagen extracting solution hydrogel and a preparation method thereof. The methodcomprises the following steps that a human mesenchymal stem cell extracellular matrix-derived composite collagen extracting solution is dissolved into a buffer solution to obtain a protein solution with the mass concentration of 0.1-100 mg / mL; natural molecules and artificially synthesized molecules are added into the protein solution, and uniform mixing is conducted to obtain a mixed solution, wherein the use final concentration of the artificially synthesized molecules is 0-100 mg / mL, and the use final concentration of the natural molecules is 0-100 mg / mL; a cross-linking activating agent isadded into the mixed solution, and uniform mixing is conducted to obtain a cross-linking reaction solution, wherein the use final concentration of the cross-linking activating agent is 1-30 mg / mL; and the cross-linking reaction liquid is purified to obtain the composite collagen extracting solution hydrogel. According to the hydrogel and the method, the gelling time is short, the biocompatibilityis good, the stability is good, and the injectability is achieved.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of medical biomaterials, in particular to a hydrogel based on human mesenchymal stem cell extracellular matrix derived composite collagen extract and a preparation method thereof. Background technique [0002] Hydrogel has a highly hydrophilic three-dimensional network structure and can maintain a high water content (70-90%). In addition, hydrogels not only provide structural support for cell attachment, but also provide a variety of biochemical and biophysical factors to regulate cell behavior. Injectable hydrogels are one of the recent focuses, and by being applied directly on the desired site in a minimally invasive manner, possible infection risks during surgery can be minimized with less scarring and / or pain, and allow Clinical application is performed in an outpatient setting rather than an operating room, thereby minimizing healthcare costs while maximizing availability. Fu...

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Problems solved by technology

Existing human collagen hydrogels have poor mechanical strength

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