Plant essential oil with broad-spectrum bactericidal effect
A technology of essential oils and bacteria, applied in the field of plant essential oils
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Embodiment 1
[0031] Embodiment 1, the steam distillation method extracts the essential oil of Cymbidium japonica
[0032] (1) Weigh 100g of the freesia flower, put it into a 1L round bottom flask with a funnel, and then add distilled water to submerge the freesia flower material (about 200-300ml distilled water) (the total volume of the flower and distilled water cannot exceed the volume of the round bottom flask. 2 / 3, in case the material is flushed out of the bottle due to boiling during distillation).
[0033] (2) Add about 4 mL of an appropriate amount of organic solvent n-hexane into the oil-water separator, and heat for about 4 hours to complete the distillation.
[0034] (4) Leave it to stand for about 20 minutes. After the essential oil is dissolved in the organic solvent (n-hexane), take the upper organic solvent in the oil-water separator, put it into a collection bottle cleaned by ultrasonic waves, and then remove the organic solvent through natural volatilization to finally obt...
Embodiment 2
[0035] Embodiment 2, research on the antibacterial activity of Fragrant Snowberry essential oil
[0036] 1 Preparation of culture medium
[0037] Bacterial culture uses LB medium: to prepare per liter of medium, add: tryptone 10g, yeast extract 5g, sodium chloride 10g to 950mL of deionized water, make the volume to 1000mL and shake the container until the solute is dissolved. Use 1mol / L NaOH to adjust the pH to 7.0, and then sterilize with steam and high pressure steam at 121°C for 20 minutes after dispensing. Solid medium: Add 15g of agar powder on the basis of liquid medium, and after aliquoting, sterilize under high pressure steam at 121°C for 20 minutes.
[0038] YPD medium is used for fungal culture: dissolve 10g of yeast extract and 20g of peptone in 900mL of water, and add 20g of agar powder to make a plate. After packaging, sterilize under high-pressure steam at 121°C for 20 minutes, and add 100 mL of prepared 20% glucose solution when used.
[0039] Bottom agar: Me...
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