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Bacillus licheniformis and application thereof

A technology of Bacillus licheniformis and bacterial agent, which is applied in the field of microorganisms to achieve the effect of effective control of cyanobacteria blooms

Active Publication Date: 2021-03-16
碧沃丰生物科技(广东)股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, a large number of studies have shown that the interaction between algae and bacteria is an important reason for the disappearance of cyanobacteria blooms. Therefore, the isolation and screening of algae-lytic bacteria that can inhibit cyanobacteria blooms in water bodies has aroused extensive attention from experts and scholars in the field of environmental protection at home and abroad. At present, more and more researchers have conducted research on the screening, algicidal characteristics, and physiological and biochemical aspects of algicidal bacteria under laboratory conditions. research still has some limitations

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  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] In this example, 100 mL of fresh Anabaena bloom liquid was treated, the concentration of chlorophyll a was 959.70±48.23 μg / L, and the pH value was 7.0. The steps of specific examples are as follows: firstly, Bacillus licheniformis BWFA55 was cultivated to the logarithmic phase (24 h) at a temperature of 30 °C and a shaker speed of 150 r / min, and then 1 Inoculate the algae liquid with mL logarithmic phase bacterial liquid, use sterile water as blank control, take a sample to determine the initial chlorophyll a concentration is 961.96±57.43 μg / L, place at 28 ℃, light intensity 2500 lux, light cycle 12 h:12 h in a light incubator for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. The concentration of chlorophyll a in the algal liquid treated by the above method was 3472.59±116.71 μg / L at 168 hours, and the concentration of chlorophyll a in the CK group was 4124.56±179.38 μg / L, and the algae inhibition rate was 15.62±6.48%...

Embodiment 2

[0064] The difference between this example and Example 1 lies in that the concentration of chlorophyll a in the treated Anabaena liquid is different, and the ratio of bacteria and algae is different. In this example, 100 mL of fresh Anabaena bloom liquid was treated, the concentration of chlorophyll a was 953.24±49.76 μg / L, and the pH value was 7.0. The steps of specific examples are as follows: firstly, Bacillus licheniformis BWFA55 was cultivated to the logarithmic phase (24 h) at a temperature of 30 °C and a shaker rotation speed of 150 r / min, and then the bacteria-algae ratio was 1:20, and the 5 mL of logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control. The initial chlorophyll a concentration was determined to be 961.96±57.43 μg / L by sampling. Placed at 28°C, light intensity 2500 lux, and light cycle for 12 h: The 12-h light incubator was cultured statically, and samples were taken every 24 h to determine t...

Embodiment 3

[0066] This example differs from Example 1 and Example 2 in that the concentration of chlorophyll a in the treated anabaena liquid is different, and the ratio of bacteria and algae to bacteria is different. In this example, 100 mL of fresh Anabaena bloom liquid was treated, the concentration of chlorophyll a was 955.28±36.00 μg / L, and the pH value was 7.0. The steps of the specific example are as follows: firstly, Bacillus licheniformis BWFA55 was cultivated to the logarithmic phase (24 h) at a temperature of 30 °C and a shaker rotation speed of 150 r / min, and then the bacteria-algae ratio was 1:10, and the 10 mL of logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control. The initial chlorophyll a concentration was determined to be 961.96±57.43 μg / L by sampling, placed at 28 °C, light intensity 2500 lux, and light cycle for 12 h: The 12-h light incubator was cultured statically, and samples were taken every 24 h to...

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Abstract

The invention discloses bacillus licheniformis and an application thereof. The bacillus licheniformis is named as bacillus licheniformis BWFA55, the preservation unit is Guangdong Province Microbial Culture Collection Center (GDMCC), the address is 5th floor, building 59, courtyard 100, Xianlie Middle Road, Guangzhou, the preservation date is December 8th, 2020, and the preservation number is GDMCC No: 61354. The bacillus licheniformis is applied to killing one or more harmful algae including anabaena flosaquae, microcystis aeruginosa and aphanizomenon flosaquae. The bacillus licheniformis isused for preventing water bloom and red tide. The bacillus licheniformis is applied to water environment treatment in aquaculture.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of bacillus licheniformis and its application. Background technique [0002] In recent years, with the rapid economic development and the continuous improvement of the quality of life, more and more nutrient-rich and complex production and domestic wastewater flow into rivers and lakes. The wastewater rich in nitrogen, phosphorus, carbon and other elements makes the biomass in the water body, especially the explosive growth of cyanobacteria, which seriously damages the ecological environment of the water body and even threatens human health. [0003] Anabaena flosaquae (Anabaena flosaquae) is a common dominant species in cyanobacterial blooms except Microcystis, which has strong nutrient competition. At the same time, Anabaena blooms can also secrete algal toxins, which have strong neurotoxicity and will directly or indirectly cause serious damage to organisms an...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N25/12A01P13/00C12R1/10C02F3/34C02F103/20
CPCA01N25/12A01N63/22C02F3/34C02F2103/20C12N1/20
Inventor 杨敏志范德朋胡亚冬雷明科陈倩瑜
Owner 碧沃丰生物科技(广东)股份有限公司
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