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Use of sulfitobacterr sp. in inducing adhesion of young mytilus coruscus

A technology of sulfite bacteria and thick-shelled mussels, which is applied in the field of marine microbial technology, and can solve the problems that natural seed cannot meet the needs of the development of thick-shelled mussels breeding industry

Active Publication Date: 2021-03-09
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, due to the over-exploitation of fishery resources, natural seedlings cannot meet the needs of the development of the mussel culture industry.

Method used

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  • Use of sulfitobacterr sp. in inducing adhesion of young mytilus coruscus
  • Use of sulfitobacterr sp. in inducing adhesion of young mytilus coruscus
  • Use of sulfitobacterr sp. in inducing adhesion of young mytilus coruscus

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Experimental program
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Embodiment

[0024] The induction experiment of deep-sea sulfite bacilli of the present embodiment attaching to thick-shell mussel juveniles:

[0025] The main steps are to inoculate the isolated and purified pure sulfite bacteria strains into seawater 2216E liquid medium for expanded cultivation, obtain bacterial precipitates after centrifugation, wash with sterilized seawater and make suspended bacterial liquid, and repeat the experiment many times. Finally, the obtained bacterial liquid was diluted and stained with acridine orange, and 10 points were randomly selected under a fluorescent microscope at 1000 times for counting, and the total density of the bacterial liquid was determined to be 4×10 9 cells / mL. Put a sterilized glass slide in each petri dish, add sterilized seawater and bacterial solution to the petri dish, so that the initial density of bacteria is 1.0×10 6 cells / mL, 1.0×10 7 cells / mL, 1.0×10 8 cells / mL and 5×10 8 cells / mL. Incubate at 18°C ​​in the dark for 48 hour...

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Abstract

The invention provides use of sulfitobacter sp. in inducing adhesion of young mytilus coruscus. Sulfitobacter sp.G213-c has the preservation number of CCTCC NO:M 2019806. The invention discloses use of a microbial film formed by the sulfitobacter sp. in inducing adhesion of young mytilus coruscus. An induction process comprises the following steps: separating and purifying sulfitobacter sp. to obtain purebred sulfitobacter sp.; carrying out inoculated culture on the purebred sulfitobacter sp., and precipitating and washing to obtain a suspended bacteria solution; and adding sterilized seawaterfor culturing to obtain a microbial film and performing an induction culture on glass slides attached with the microbial film and the young mytilus coruscus. Therefore, the microbial film formed by the sulfitobacter sp. can induce adhesion of young mytilus coruscus, an induction activity of the microbial film with different bacterium initial densities on the young mytilus coruscus is different, and the sulfitobacterr sp. has important significance on artificial culture of the mytilus coruscus.

Description

technical field [0001] The invention belongs to the technical field of marine microbes, and relates to the application of a strain of deep-sea sulfite bacteria in inducing the attachment of mussel juveniles, in particular to the isolation, purification, identification and identification of a strain of deep-sea sulfite bacteria Sulfitobacter sp.G213-c. Preservation, and the effect of microbial film formed under different initial bacterial densities on the attachment of juvenile mussel spat. Background technique [0002] Thick-shelled mussel (Mytilus coruscus), belonging to the mollusca (Mollusca), bivalves (Bivalvia), mytilodia (Mytilodia), mytilidae (Mytilidae), is an important marine shellfish culture species in my country, distribution Along the coasts of the Yellow Sea, the Bohai Sea and the East China Sea. Thick-shelled mussels have high nutritional value and have broad breeding prospects and market development potential. In the life history of thick-shelled mussels, in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K61/54C12R1/01
CPCC12N1/20A01K61/54Y02A40/81
Inventor 梁箫胡晓梦彭莉华竹攸汀杨金龙魏玉利方家松
Owner SHANGHAI OCEAN UNIV
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