Novel cell membrane specific formaldehyde fluorescent probe as well as preparation method and application thereof

Abstract

The invention relates to a novel cell membrane specific fluorescent probe as well as a preparation method and application thereof; particularly, the novel cell membrane specific fluorescent probe canbe used for measuring, detecting or screening formaldehyde and living cell fluorescence imaging, and particularly can be applied to distinguishing and detecting endogenous formaldehyde and exogenous formaldehyde and imaging. The probe can realize at least one of the following technical effects: targeting and positioning cell membranes, distinguishing and detecting endogenous formaldehyde and exogenous formaldehyde, identifying formaldehyde with high selectivity, quickly responding to formaldehyde, sensitively analyzing formaldehyde, detecting formaldehyde under physiological level conditions,and being simple to synthesize and stable in property.

Description

technical field [0001] The invention belongs to the field of fluorescent probes, in particular to a formaldehyde fluorescent probe based on naphthalimide compounds and its application in measuring, detecting or screening formaldehyde and live cell fluorescence imaging methods; the invention also provides the prepared Methods for fluorescent probes. Background technique [0002] Formaldehyde is the simplest aldehyde substance and a typical active carbonyl substance; formaldehyde is also one of the important raw materials for the industrial production of urea-formaldehyde resin and phenolic resin, and is extremely important for the clothing textile industry and furniture manufacturing industry. At the same time, formaldehyde is also used in the production of disinfectants, preservatives, pesticides and other industries. At this stage, the human demand for formaldehyde is still very large. [0003] Formaldehyde has long been recognized as a human toxin and carcinogen, release...

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Problems solved by technology

[0005] In recent years, the reported methods for detecting formaldehyde include spectrophotometric colorimetry, high performance liquid chromatography, chemiluminescence analysis, photochemical sensing, etc., but these methods are complicated and cannot detect formaldehyde in vivo, so fluorescent probes It has become the focus of researchers because of its unique advantages such as high selection, ultra-sensitivity, and simple synthesis.

There are still some defects in the reported fluorescent probe analysis methods, such as low sensitivity, poor selectivity, complex synthesis, long response time, etc. Other active carbonyl substances in the living body are similar to formaldehyde, which will pose a potential problem for the detection of formaldehyde. interference, and the currently reported fluorescent probes cannot distinguish between endogenous and exogenous formaldehyde. Therefore, the development of fast, highly sensitive and highly selective fluorescent probes, especially the ability to distinguish between endogenous and exogenous formaldehyde Fluorescent probes have become an urgent problem for those skilled in the art

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