Use of bile acid complex microbial inoculant in preparing pig feed additive
A pig feed additive and compound bacterial agent technology, applied in the field of poultry and livestock breeding, can solve the problems of reduced body self-purification ability, reduced piglet survival rate, and weakened piglet physique, so as to restore liver and intestine function, restore body self-purification ability, and increase milk production Effect
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Embodiment 1
[0033] 1. Screening and identification of Parabacteroides distirii LCG-06
[0034] 1. Screening of Parabacteroides distirii LCG-06
[0035] Take intestinal feces from the intestines of pigs, mix 10g of feces and 100mL sterile water, filter to remove impurities, and make a feces dilution, and then continue to add sterile water to the dilution to prepare 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 diluent; then take 10 -2 、10 -3 、10 -4 、10 -5 Spread the dilution on soybean casein agar medium (TSA) in a medium containing 80% N 2 , 10% CO 2 and 10%H 2 Under an anaerobic environment, cultured at 37°C for 4 days, selected obvious colonies and purified them several times to obtain a single colony, which was named LCG-06, and then placed in soybean casein agar slant medium for refrigerated storage. The formula of the TSA medium is: tryptone 15.0g, soytone 5.0g, sodium chloride 5.0g, agar 15.0g, sterile water 1000mL, pH 7.3±0.2.
[0036] Such as figure 1 As shown, the biological cha...
Embodiment 2
[0066] There are 200 sows in a farm in Guangxi, and the piglets in the farrowing room have diarrhea a few days before birth, such as image 3 and Figure 4 As shown, the feces excreted by piglets are yellow or white gruel-like, with no specific foul smell, too many air bubbles, and no visible blood components. The veterinarian has diagnosed that the piglets are caused by maternal toxin infection and insufficient milk. Sexual diarrhea. These piglets often vomit before diarrhea, and the vomit is yellow-white curd mass, and the vomiting will stop when diarrhea begins, and during the diarrhea process, the piglets are not in good spirits, and the body temperature is kept at about 40 ° C. In addition, after diarrhea, the piglets Accelerated dehydration, coupled with less milk intake, dehydration and death of piglets ( Figure 5 ), the dead piglets were dissected and found that the liver was enlarged, the kidneys were deformed, and the intestines were congested ( Figure 6 ).
[...
Embodiment 3
[0069] A pig breeding factory in Laizhou, Shandong, with a breeding scale of 536 pigs. During the breeding period, it was found that pregnant sows had tear spots ( Figure 8 ), and 30 of them were selected for the effect experiment of bile acid compound bacterial agent. Pig tear spots are only the appearance symptoms of sows. In fact, they are poisoning and liver damage caused by infection caused by viruses or bacteria in pigs, abuse of antibiotics, etc., and cause the decline of autoimmunity to reflect in the eyes of pigs.
[0070] The 30 sows were reared separately, and the bile acid compound bacterial agent was evenly mixed into the feed with the amount of 2L / ton of feed, 3 times a day, and fed continuously for 20 days. Such as Figure 8 As shown, after 20 days, the tear spots of sows were significantly reduced, and some even disappeared. After 20 days, the bile acid compound bacterial agent was evenly mixed into the feed at an amount of 1L / ton of feed to consolidate the ...
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