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Use of bile acid complex microbial inoculant in preparing pig feed additive

A pig feed additive and compound bacterial agent technology, applied in the field of poultry and livestock breeding, can solve the problems of reduced body self-purification ability, reduced piglet survival rate, and weakened piglet physique, so as to restore liver and intestine function, restore body self-purification ability, and increase milk production Effect

Active Publication Date: 2021-02-26
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially for sows before delivery, problems such as the breeding environment will cause the accumulation of toxins in the sows, and then metabolic diseases and poisoning diseases will occur, resulting in a decrease in the self-purification ability of the body, which will also affect the physical weakness of the piglets produced by the sows. Problems such as diarrhea; diarrhea in piglets can lead to lesions in organs such as the liver and kidneys, resulting in a decrease in the survival rate of piglets
At present, antibiotics are mostly used to solve problems such as endotoxin accumulation and diarrhea in sows and piglets, but antibiotics will cause the body to develop drug resistance, which is not conducive to pig breeding

Method used

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  • Use of bile acid complex microbial inoculant in preparing pig feed additive
  • Use of bile acid complex microbial inoculant in preparing pig feed additive
  • Use of bile acid complex microbial inoculant in preparing pig feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Screening and identification of Parabacteroides distirii LCG-06

[0034] 1. Screening of Parabacteroides distirii LCG-06

[0035] Take intestinal feces from the intestines of pigs, mix 10g of feces and 100mL sterile water, filter to remove impurities, and make a feces dilution, and then continue to add sterile water to the dilution to prepare 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 diluent; then take 10 -2 、10 -3 、10 -4 、10 -5 Spread the dilution on soybean casein agar medium (TSA) in a medium containing 80% N 2 , 10% CO 2 and 10%H 2 Under an anaerobic environment, cultured at 37°C for 4 days, selected obvious colonies and purified them several times to obtain a single colony, which was named LCG-06, and then placed in soybean casein agar slant medium for refrigerated storage. The formula of the TSA medium is: tryptone 15.0g, soytone 5.0g, sodium chloride 5.0g, agar 15.0g, sterile water 1000mL, pH 7.3±0.2.

[0036] Such as figure 1 As shown, the biological cha...

Embodiment 2

[0066] There are 200 sows in a farm in Guangxi, and the piglets in the farrowing room have diarrhea a few days before birth, such as image 3 and Figure 4 As shown, the feces excreted by piglets are yellow or white gruel-like, with no specific foul smell, too many air bubbles, and no visible blood components. The veterinarian has diagnosed that the piglets are caused by maternal toxin infection and insufficient milk. Sexual diarrhea. These piglets often vomit before diarrhea, and the vomit is yellow-white curd mass, and the vomiting will stop when diarrhea begins, and during the diarrhea process, the piglets are not in good spirits, and the body temperature is kept at about 40 ° C. In addition, after diarrhea, the piglets Accelerated dehydration, coupled with less milk intake, dehydration and death of piglets ( Figure 5 ), the dead piglets were dissected and found that the liver was enlarged, the kidneys were deformed, and the intestines were congested ( Figure 6 ).

[...

Embodiment 3

[0069] A pig breeding factory in Laizhou, Shandong, with a breeding scale of 536 pigs. During the breeding period, it was found that pregnant sows had tear spots ( Figure 8 ), and 30 of them were selected for the effect experiment of bile acid compound bacterial agent. Pig tear spots are only the appearance symptoms of sows. In fact, they are poisoning and liver damage caused by infection caused by viruses or bacteria in pigs, abuse of antibiotics, etc., and cause the decline of autoimmunity to reflect in the eyes of pigs.

[0070] The 30 sows were reared separately, and the bile acid compound bacterial agent was evenly mixed into the feed with the amount of 2L / ton of feed, 3 times a day, and fed continuously for 20 days. Such as Figure 8 As shown, after 20 days, the tear spots of sows were significantly reduced, and some even disappeared. After 20 days, the bile acid compound bacterial agent was evenly mixed into the feed at an amount of 1L / ton of feed to consolidate the ...

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Abstract

The invention discloses use of a bile acid complex microbial inoculant in preparing a pig feed additive. The bile acid complex microbial inoculant comprises a parabacteroides distasonis suspension, abacteroides thetaiotaomicron suspension, bile acid and eucalyptus polybractea essential oil in a volume-to-mass ratio of (2-3):(2-4):(2-3):1. The parabacteroides distasonis suspension is prepared fromparabacteroides distasonis LCG06 with the preservation number of CGMCC No.20820. The bacteroides thetaiotaomicron suspension is prepared from bacteroides thetaiotaomicron GL02 with the preservation number of CGMCC No.20818. The complex microbial inoculant has natural components, is free of toxic and side effects, can remarkably improve and recover intestinal health of sows, improves nonspecific immunity of the sows, promotes discharge of vivotoxin, increases the lactation yield of the sows, also enhances physique of piglets, increases a survival rate of the piglets, repairs liver and gall injuries of the piglets and improves breeding income.

Description

technical field [0001] The invention belongs to the field of poultry breeding, and in particular relates to the application of a bile acid compound bacterial agent in preparing pig feed additives. [0002] technical background [0003] Nowadays, pig breeding is generally large-scale farming. In order to reap higher profits, farmers will choose to over-breed and feed excessive feed. These problems will cause chaos in the pig breeding environment and affect the health of the pigs. . At present, the main problems of pig farming are concentrated in the toxic state of the body, as well as malnutrition, immunosuppression, and inflammation. Especially for sows before delivery, problems such as the breeding environment will cause the accumulation of toxins in the sows, and then metabolic diseases and poisoning diseases will occur, resulting in a decrease in the self-purification ability of the body, which will also affect the physical weakness of the piglets produced by the sows. D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K10/18A23K20/105A23K10/30C12N1/20C12N1/02C12R1/01
CPCA23K50/30A23K10/18A23K20/105A23K10/30C12N1/20C12N1/02Y02P60/87
Inventor 曹爱智娄倩倩籍立民
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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