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A cell preservation solution for rapid cell immunohistochemistry and its preparation method and application

A technology of cell immunity and preservation solution, which is applied in the field of cytopathological detection, can solve the problems of cell surface deformation, protein mucus agglomeration deposition, and cell clumps, so as to shorten the reaction time, reduce the pain of patients, and improve the detection efficiency.

Active Publication Date: 2022-02-22
河南赛诺特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Immunohistochemical technology of living tissue in pathological diagnosis has been quite perfect, but cell immunohistochemical technology is not particularly mature
Mainly there are two problems: the one, how to properly fix, keep the morphological structure of cell intact as far as possible, prevent from coming off
However, the current sample preservation solution often uses high concentrations of alcohols, aldehydes, and normal saline to preserve cell specimens, which will cause cell clusters, protein mucus agglomeration and deposition, poor sample transfer and observation effects, and easily lead to cell loss. Surface deformation makes it difficult to accurately observe the shape of target cells, which affects the interpretation of cell staining results
In addition, most of the cell preservation solutions seen so far are only used for TCT detection, and a small number of products can be used for cell immunohistochemical detection, but cell repair operations are still required; this invisibly weakens the timeliness of pathological test results

Method used

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  • A cell preservation solution for rapid cell immunohistochemistry and its preparation method and application
  • A cell preservation solution for rapid cell immunohistochemistry and its preparation method and application
  • A cell preservation solution for rapid cell immunohistochemistry and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of Cell Preservation Solution 1 for Rapid Immunohistochemistry

[0044] For the cell preservation solution 1 for rapid immunohistochemistry provided in this example, weigh or measure each component according to the following table:

[0045] Table 1 Formula of Cell Preservation Solution 1

[0046]

[0047]

[0048] In the above formula, "w / v" or "v / v", "v" in the denominator refers to the total volume of the prepared cell preservation solution; "w" or "v" in the numerator refers to the corresponding group points of mass or volume.

[0049] The preparation method of cell preservation solution 1 for rapid immunohistochemistry provided in this example is as follows:

[0050] 1) According to the formula shown in Table 1, add Tris buffer and NaCl, KCl, MgCl in double distilled water 2 , PEG4000, TritonX-100, EDTA.2NA, TCEP, and get solution A after mixing;

[0051] 2) Add fixative ethylene glycol and methanol to solution A, and mix well to obtain...

Embodiment 2

[0057] Example 2 Preparation of Cell Preservation Solution 2 for Rapid Immunohistochemistry

[0058] For the cell preservation solution 2 for rapid immunohistochemistry provided in this example, weigh or measure each component according to the following table:

[0059] Formulation of table 2 cell preservation solution 2

[0060]

[0061]

[0062] In the above formula, "w / v" or "v / v", "v" in the denominator refers to the total volume of the prepared cell preservation solution; "w" or "v" in the numerator refers to the corresponding group points of mass or volume.

[0063] The preparation method of cell preservation solution 1 for rapid immunohistochemistry provided in this example is as follows:

[0064] 1) According to the formula shown in Table 1, add phosphate buffer, NaCl, KCl, PEG4000, EDTA.2NA and TCEP into double distilled water, and mix well to obtain solution A;

[0065] 2) Add fixative ethylene glycol and ethanol to solution A, and mix to obtain solution B; ...

Embodiment 3

[0071] Example 3 Preparation of Cell Preservation Solution 3 for Rapid Immunohistochemistry

[0072] For the cell preservation solution 2 for rapid immunohistochemistry provided in this example, weigh or measure each component according to the following table:

[0073] Table 3 Formula of Cell Preservation Solution 3

[0074]

[0075]

[0076] In the above formula, "w / v" or "v / v", "v" in the denominator refers to the total volume of the prepared cell preservation solution; "w" or "v" in the numerator refers to the corresponding group points of mass or volume.

[0077] The preparation method of cell preservation solution 1 for rapid immunohistochemistry provided in this example is as follows:

[0078] 1) According to the formula shown in Table 3, add potassium phosphate buffer solution and NaCl, KCl, PEG4000, NH 4 CL, sodium citrate, and sodium mercaptosulfonate were mixed to obtain solution A;

[0079] 2) Add fixatives such as isopropanol and ethanol to solution A, an...

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Abstract

The invention relates to a cell preservation solution for rapid cell immunohistochemistry, a preparation method and application thereof. The cell preservation solution provided by the present invention is mainly composed of buffer, erythrocyte lysis reagent, osmotic pressure maintenance agent, cell shape maintenance reagent, fixative, preservative, mucus treatment reagent, anti-aggregation reagent and cell antigen treatment for immunohistochemistry Reagent composition; wherein, the reagents used for cell immunohistochemical cell antigen treatment include formalin and zinc salicylate, and the synergy of the two can effectively catalyze the immunohistochemical reaction, so that the cell antigen does not need to be repaired, shortening The reaction time of cell immunohistochemical detection improves the detection efficiency and enhances the timeliness of pathological diagnosis. The cell preservation solution provided by the present invention can be applied to preserve or collect various cell samples such as cervical exfoliated cells, pleural effusion, alveolar lavage fluid, cerebrospinal fluid, sputum and urine. At the same time, the present invention provides a preparation method of the above preservation solution.

Description

technical field [0001] The invention belongs to the field of cytopathological detection, and in particular relates to a cell preservation solution for rapid cell immunohistochemistry, a preparation method and application thereof. Background technique [0002] Immunohistochemical technology of living tissue in pathological diagnosis has been quite perfect, but cell immunohistochemical technology is not particularly mature. Mainly there are two problems: the one, how to fix properly, keep the morphological structure of cell intact as far as possible, prevent from coming off. The second is how to fully expose the cell antigenic determinants, improve the permeability of the cell membrane, and make it easier for the antibody macromolecules to enter the cell, so as to facilitate the better combination of the antibody and the antigen. Therefore, how to preserve cell samples and how to handle cells to fully expose antigenic determinants is the key to the success of cell immunohisto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02G01N33/569
CPCA01N1/0231A01N1/021G01N33/56966
Inventor 米贯勋牛银银杨潇燕崔红米李三华刘文弟齐华
Owner 河南赛诺特生物技术有限公司
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