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Preparation method and application of myrothamnus flabellifolia extract

A technology of extract and miro, applied in the field of preparation of miro extract, can solve problems such as low extraction rate of flavonoids, and achieve excellent effects, excellent anti-aging and anti-oxidation effects

Pending Publication Date: 2021-02-23
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But there are few reports on the extraction method of Milo in the prior art
Simultaneously, due to the particularity of Milo wood's own structure, the existing extraction process for flavonoids is not suitable for extracting the flavonoids in Milo wood, and the flavonoid extraction rate is generally low (extracted flavonoid content is 9-15 %), for the improvement to above-mentioned situation, need provide a kind of method aiming at extracting flavonoids from Milo

Method used

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  • Preparation method and application of myrothamnus flabellifolia extract
  • Preparation method and application of myrothamnus flabellifolia extract
  • Preparation method and application of myrothamnus flabellifolia extract

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, milo wood extract

[0031] Described preparation method is specifically:

[0032] A) the Milo wood is pulverized to obtain the first powder after passing through a 40 mesh sieve;

[0033] B) Take the first powder in step A), add petroleum ether in an amount 8 times the total amount of the above powder and soak overnight to obtain a degreasing mixture; filter the degreasing mixture, and dry the obtained filter residue to obtain a second powder;

[0034] C) Mix the second powder of step B) with the extraction solvent in a weight ratio of 1:45, extract at 3°C ​​for 1 hour, extract with 380W ultrasonic at 45°C for 1 hour, heat in a boiling water bath for 10 minutes to inactivate the enzyme, and extract the enzyme after inactivating The liquid was centrifuged at 4500rmp in a centrifuge for 30min, and the filter residue was removed to obtain a supernatant; the supernatant was preliminarily purified through a 400nm ceramic membrane to obtain a filtrate; the fi...

Embodiment 2

[0036] Embodiment 2, milo wood extract

[0037] Described preparation method is specifically:

[0038] A) the Milo wood is pulverized to obtain the first powder after passing through a 40 mesh sieve;

[0039] B) Take the first powder in step A), add petroleum ether in an amount 10 times the total amount of the above powder and soak overnight to obtain a degreasing mixture; filter the degreasing mixture, and dry the obtained filter residue to obtain a second powder;

[0040] C) Mix the second powder of step B) with the extraction solvent in a weight ratio of 1:60, extract at 3°C ​​for 1.5h, and at 45°C with 380W ultrasonic extraction for 1.5h, heat in a boiling water bath for 15min to inactivate the enzyme. The extract was centrifuged at 4500rmp for 30min in a centrifuge, and the filter residue was removed to obtain a supernatant; the supernatant was preliminarily purified through a 400nm ceramic membrane to obtain a filtrate; the filtrate was concentrated under reduced pres...

Embodiment 3

[0042] Embodiment 3, milo wood extract

[0043] Described preparation method is specifically:

[0044] A) the Milo wood is pulverized to obtain the first powder after passing through a 40 mesh sieve;

[0045] B) Take the first powder in step A), add petroleum ether in an amount 10 times the total amount of the above powder and soak overnight to obtain a degreasing mixture; filter the degreasing mixture, and dry the obtained filter residue to obtain a second powder;

[0046]C) Mix the second powder of step B) with the extraction solvent in a weight ratio of 1:50, extract at 5°C for 1 hour, extract with 380W ultrasonic at 50°C for 1 hour, heat in a boiling water bath for 15 minutes to inactivate the enzyme, and extract The liquid was centrifuged at 4500rmp in a centrifuge for 30min, and the filter residue was removed to obtain a supernatant; the supernatant was preliminarily purified through a 400nm ceramic membrane to obtain a filtrate; the filtrate was concentrated under re...

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Abstract

The invention discloses a preparation method of a myrothamnus flabellifolia extract, and relates to the technical field of cosmetics. The preparation method comprises the following steps: A) crushingmyrothamnus flabellifolia to obtain first powder; B) degreasing, filtering and drying the first powder to obtain second powder; and C) extracting the second powder by adopting an ultrasonic-assisted compound enzyme mode, and conducting deactivating and purifying to obtain the myrothamnus flabellifolia extract. According to the method of the invention, degreasing, ultrasonic-assisted compound enzyme extraction and purification are adopted, the flavone content in the obtained myrothamnus flabellifolia extract can reach 28.5% at most, the extraction rate reaches 86.0% or above, and the myrothamnus flabellifolia extract has remarkable antioxidant and anti-aging functions.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a preparation method and an application of Milo wood extract. Background technique [0002] Skin aging is a gradual physiological process, and it is also the result of the combined effects of various factors such as genetics, environment, and social psychology. The main manifestations are thinning of the epidermis and dermis, rough skin, dryness, loss of luster, loss of elasticity, decrease of water content and blood flow inside the skin, and decrease of secretion of sweat glands and sebaceous glands, etc., resulting in slower epidermal renewal, weakened barrier function, and keratinization. Decreased cell viability and weakened repair ability of the epidermis after injury. Regarding the anti-aging mechanism of anti-aging functional ingredients in cosmetics, the current academic circle generally agrees with the theory of "free radical aging" proposed by Denham Harm...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/08
CPCA61K8/9789A61Q19/08A61K2800/82A61K2800/805A61K2800/782
Inventor 王丽华孙怀庆孙云起曾令椿郭朝万胡露
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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