Magnetic bead method nucleic acid extraction equipment

Abstract

The invention discloses magnetic bead method nucleic acid extraction equipment, which comprises a bottom plate, a vertical plate arranged on the bottom plate and an extraction pipe arranged on the bottom plate, a mounting groove is formed in the bottom plate, the extraction pipe is inserted into the mounting groove, a plurality of liquid outlet holes are formed in the bottom of the extraction pipe, a liquid inlet pipe is arranged on the side wall of the extraction pipe, and a sealing plate matched with the liquid outlet hole is further arranged at the bottom of the extraction pipe; wherein a first sleeve is arranged in the extraction pipe in a penetrating manner, a first push plate is arranged on the first sleeve, a plurality of through grooves are formed in the first push plate, and a plurality of first baffle plates matched with the through grooves are arranged on the first push plate; a magnetic rod is arranged on the vertical plate, and a driving motor used for driving the first sleeve to rotate is further arranged on the vertical plate.

Description

technical field [0001] The invention belongs to the technical field of nuclide extraction, and in particular relates to a magnetic bead method nucleic acid extraction equipment. Background technique [0002] In the nucleic acid extraction operation of the magnetic bead method, whether the mixing of the magnetic beads and the free nucleic acid is sufficient, and whether the magnetic rod is completely adsorbed to the magnetic beads are crucial to the nucleic acid extraction effect, especially the magnetic bead mixing operation and magnetic bead adsorption of large-volume samples. Particularly important. For the routine small-capacity nucleic acid extraction work, when extracting nucleic acid, the magnetic rod and the free nucleic acid are mixed evenly through the up and down reciprocating motion of the magnetic rod, the rotary motion of the magnetic rod sleeve, and the suction and discharge of the pipette. The magnetic beads adsorb nucleic acid, and then the magnetic rod adso...

AI Technical Summary

Problems solved by technology

[0003] For the work of extracting large sample volume, when extracting nucleic acid, after adding lysate, buffer and magnetic beads, the total liquid volume is larger, and it is necessary to use Large-volume sample tubes make it difficult to fully mix the large-volume sample liquid with magnetic beads, and it is difficult to completely extract nucleic acid with ordinary nucleic acid extraction instruments: the extraction of nucleic acid is not complete or the extraction time needs to be increased for repeated mixing and extraction Work, affecting the extraction rate and extraction efficiency

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