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Cryoprotectant capable of being directly transfused back for stem cells and related products thereof

A technology for stem cells and cryopreservation solution, applied in the field of cryopreservation agents, can solve the problems of self-renewal of cultured stem cells and uncertain factors in the differentiation process, avoid uncertain factors and contamination of cultured cells, avoid inducing immune responses, The effect of increasing production

Inactive Publication Date: 2021-02-12
MEI HOSPITAL UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the cryoprotectant is poorly selected, such as the use of animal-derived cryopreservation agents, it may bring uncertainties to the self-renewal and differentiation process of cultured stem cells

Method used

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  • Cryoprotectant capable of being directly transfused back for stem cells and related products thereof
  • Cryoprotectant capable of being directly transfused back for stem cells and related products thereof
  • Cryoprotectant capable of being directly transfused back for stem cells and related products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Cryopreservation, recovery and characteristic detection of umbilical cord mesenchymal stem cells

[0040] 1. Experimental materials

[0041] Umbilical cord mesenchymal stem cells, DMSO, compound electrolyte injection, 5% glucose injection, 20% human serum albumin injection, low molecular weight dextran injection, Thermo CO 2 Incubator, Thermo centrifuge, biological safety cabinet, Accuri C6plus flow cytometer, liquid nitrogen tank.

[0042] 2. Test method

[0043] Example 1

[0044] Preparation of cryopreservation solution: in parts by volume, measure 6 parts of DMSO, 32 parts of compound electrolyte injection, 32 parts of 5% glucose injection by mass fraction, 18 parts of 20% human serum albumin injection by mass fraction, 12 parts of low-molecular-weight dextran injection, the components are mixed and shaken evenly.

[0045] The compound electrolyte injection contains 5.26g of sodium chloride, 5.02g of sodium gluconate, 3.68g of sodium acetate, 0.37g of ...

Embodiment 2

[0056] Preparation of cryopreservation solution: in parts by volume, measure 5 parts of DMSO, 30 parts of compound electrolyte injection, 30 parts of 5% glucose injection by mass fraction, 25 parts of 20% human serum albumin injection by mass fraction, 10 parts of low-molecular-weight dextran injection, the components are mixed and shaken evenly.

[0057] The compound electrolyte injection contains 5.26g of sodium chloride, 5.02g of sodium gluconate, 3.68g of sodium acetate, 0.37g of potassium chloride and 0.30g of magnesium chloride per 1000mL.

Embodiment 3

[0059] Preparation of cryopreservation solution: in parts by volume, measure 10 parts of DMSO, 35 parts of compound electrolyte injection, 35 parts of 5% glucose injection by mass fraction, 10 parts of 20% human serum albumin injection by mass fraction, 10 parts of low-molecular-weight dextran injection, the components are mixed and shaken evenly.

[0060] Other operation steps of embodiment 2 and embodiment 3 are identical with embodiment 1, also can obtain conclusion of the present invention.

[0061] Using the accelerated destruction experiment to measure the shelf life of the cryoprotectant of the present invention, the shelf life of the cryoprotectant of the present invention is measured to be 2 years, and the cryoprotectant of the present invention can be used to directly cryopreserve mesenchymal stem cells within two years , and the mesenchymal stem cells can be recovered after being cryopreserved for a certain period, and can be directly injected and used, that is, dir...

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Abstract

The invention provides a cryoprotectant capable of being directly transfused back for stem cells and related products thereof, and belongs to the field of cryopreservation protective agents. The cryoprotectant solves the problems that animal-derived raw materials are added into an existing cryoprotectant, uncertain factors are brought to the self-renewal and differentiation process of cultured cells, and the like, and the cryoprotectant used for stem cells and related products thereof and capable of being directly reinfused comprises the following components in parts by volume: 5-10 parts of dimethyl sulfoxide, 30-35 parts of a compound electrolyte injection, 30-35 parts of glucose injection, 15-20 parts of human serum albumin injection and 10-15 parts of dextran injection. The cryoprotectant has the advantages that the stem cells can be directly transfused back after being revived, cleaning is not needed, time and cost are saved, and the cell yield and cell viability can be improved.

Description

technical field [0001] The invention relates to the field of cryopreservation agents, in particular to a cryopreservation solution for stem cells and related products that can be directly reinfused. Background technique [0002] Stem cells refer to a type of cell that is capable of self-renewal, division, and differentiation into other cell types. [0003] At present, stem cell research has completed a series of safety experiments, including toxicity test, genetics test, local stimulation test, fever test and immunotoxicity test, etc. The results show that stem cells are safe and non-toxic. In addition, adult stem cells have no mutation phenomenon after multiple passages, that is to say, there is no possibility of tumorigenesis. A large amount of clinical data also shows that, except for a very small number of patients with short-term, mild fever and headache during stem cell therapy, no other significant side effects or adverse reactions have been found. [0004] The step...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K47/42A61K47/26A61K47/36A61K47/02A61K47/20A61K47/12A61K9/00A01N1/02
CPCA01N1/021A61K9/0019A61K35/28A61K47/02A61K47/12A61K47/20A61K47/26A61K47/36A61K47/42
Inventor 张顺阮春燕施晓峰蔡挺张沛瑶
Owner MEI HOSPITAL UNIV OF CHINESE ACAD OF SCI
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