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Fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin

A technology of chorionic gonadotropin and estradiol, which is applied in biological testing, measuring devices, material inspection products, etc., and can solve the problem of lack of efficient detection kits for progesterone, estradiol and β-human chorionic gonadotropin, etc. problem, achieve the effect of reducing the chance of uterine contractions or infection

Pending Publication Date: 2021-02-05
RELIA BIOTECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, there are only single detection kits for progesterone, estradiol and β-human chorionic gonadotropin, and there is a lack of kits that can simultaneously and efficiently detect progesterone, estradiol and β-human chorionic gonadotropin

Method used

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  • Fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin
  • Fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin
  • Fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Progesterone, Estradiol and β-Human Chorionic Gonadotropin Fluorescent Quantitative Detection Kit

[0025] 1. Composition and preparation method of the kit

[0026] Progesterone, estradiol and β-human chorionic gonadotropin fluorescent quantitative detection kit, including: test card, U disk with standard curve.

[0027] (1) Structure of test card

[0028] combined figure 1 and figure 2 , illustrating the structure of the test card.

[0029] The test card includes a card shell and a test strip inside the card shell.

[0030] The test strip includes a substrate 6 and a sample pad 7 , a CP pad 8 , an NC film 9 and an absorbent pad 10 pasted on the substrate in sequence. Adjacent ends of the sample pad, CP pad, NC membrane, and absorbent pad overlap each other.

[0031] The cartridge includes an upper cartridge 1 and a lower cartridge 2 that are engaged with each other. The inner surface of the lower cartridge 2 is provided with a drawer slot 5 for placing...

Embodiment 2

[0065] Embodiment 2 The effect contrast of test strip of the present invention and other test strips

[0066] Prepare control test strips 1-3.

[0067] According to the preparation method of the test strip of the present invention, prepare the control test strip 1, the difference is that the Prog antibody (purchased from Meridian, product number E82321M) in the ProgmAb labeled conjugate on the CP pad is replaced by Hytest company, the product number is Prog antibody to HPRO-2.

[0068] Prepare the contrast test strip 2 according to the preparation method of the test strip of the present invention, the difference is only that the E2 antigen whose product number is 710050 of the Medix company on the NC membrane is replaced by the E2 antigen which is numbered V56110 by the Biospacific company, and the E2 antigen on the CP pad is replaced. The E2 antibody (Hytest, 2E2) in the mAb-labeled conjugate was replaced with the E2 antibody whose product number is HM177A-1177A from Holmes ...

Embodiment 3

[0078] Embodiment 3 adopts kit of the present invention to detect concrete serum sample

[0079] According to the preparation method of the test strip of the present invention, a batch of detection kits are prepared, and the correlation comparison is carried out with the serum samples assigned by the Roche cobas e411 electrochemiluminescence automatic immunoassay system. Progesterone, estradiol and β-human chorionic gonadotropin each detect 50 samples, take the detection result of Roche cobas e411 electrochemiluminescence automatic immunoassay system as the control group, compare the detection result of the present invention and its correlation, the result is as follows Figure 6-8 . It can be seen that the detection result of the kit of the present invention is extremely accurate.

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Abstract

The invention provides a fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin, and relates to the technical field of in vitro diagnosis. The kit comprises a test strip, wherein the test strip comprises a substrate, and a sample pad, a CP pad, an NC membrane and an absorption pad which are sequentially adhered to the substrate; the CP pad is coated with a mouse anti-progesterone monoclonal antibody, a mouse anti-estradiol monoclonal antibody, a mouse anti-beta human chorionic gonadotropin monoclonal antibody and a fluorescein labeled conjugateof chicken IgY; and a detection line coated with a progesterone antigen, an estradiol antigen and a beta-HCG monoclonal antibody and a quality control line coated with a rabbit anti-chicken IgY monoclonal antibody are respectively arranged on the NC membrane. According to the kit, three early pregnancy indexes can be rapidly, accurately and quantitatively detected at the same time through one-time sampling, and a more accurate reference basis is provided for dynamically detecting early pregnancy hormone level changes and predicting the pregnancy outcome of early threatened abortion patients.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin. Background technique [0002] Progesterone, β-human chorionic gonadotropin and estradiol play an extremely important role in early pregnancy. Progesterone (abbreviated as Prog) is a steroid hormone produced by the corpus luteum in early pregnancy, and β-human chorionic gonadotrophin (abbreviated as β-HCG) is a hormone secreted by syncytiotrophoblasts. glycoprotein, and estradiol (Estrodiol, abbreviated as E2) is a natural steroidal estrogen secreted by ovarian mature follicles. [0003] In the first trimester, progesterone is mainly produced by the corpus luteum of pregnancy, and is replaced by placental syncytiotrophoblasts after 8 to 10 weeks. Therefore, the level of progesterone reflects the functions of the corpus luteum of pregnancy and the placenta. Befor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/74G01N33/577G01N33/558G01N33/58
CPCG01N33/76G01N33/743G01N33/577G01N33/558G01N33/582
Inventor 任婷尹欢朱庆平陈金龙
Owner RELIA BIOTECH JIANGSU
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