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Bacillus velezensis and use thereof in preventing and treating diseases of bergamot pears

A technology of Bacillus Velez and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of lack of new prevention and control technologies, frequent use of pesticides, excessive pesticide residues in fruits, etc., and achieve good protection and treatment effects , not easy to produce drug resistance, good protective effect

Active Publication Date: 2021-02-05
XINJIANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is a lack of safe and efficient new disease prevention and control technologies in production. A single drug for one disease is often neglected. The frequent use of pesticides and the problem of random application and abuse are prominent, which inevitably leads to increased resistance of pathogenic bacteria, excessive pesticide residues in fruits, and environmental pollution. series of questions

Method used

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  • Bacillus velezensis and use thereof in preventing and treating diseases of bergamot pears
  • Bacillus velezensis and use thereof in preventing and treating diseases of bergamot pears
  • Bacillus velezensis and use thereof in preventing and treating diseases of bergamot pears

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and screening of antagonistic bacteria

[0034](1) Isolation and purification of strains: In Korla, Xinjiang, the enzyme fermentation liquid made by farmers using watermelon, melon and other fruits and vegetables as raw materials was collected, and the fermentation bacteria in the enzyme fermentation liquid were separated by the dilution plate method. Pick a single colony according to the different shapes, colors, and sizes of the colonies grown on the plate, and after repeated streaking and purification, transfer to the slant of NA medium and store at 4°C.

[0035] (2) Preparation of the fermentation broth of the strain to be tested: Inoculate the pure culture of the strain to be tested into a 250 mL Erlenmeyer flask filled with 80 mL of NB culture liquid, and incubate at a constant temperature of 160 r / min at 28°C for 1-2 d to Bacteria solution OD 600 0.8 to 1.0.

[0036] (3) Screening of antagonistic strains of E. amylovora and E. amylovora: Ino...

Embodiment 2

[0041] Example 2 Identification of bacterial strain gjfn2

[0042] (1) Observation of strain morphology and culture characteristics

[0043] The strain gjfn2 was inoculated on NA solid medium for cultivation, and after culturing at 28°C for 24 h, it formed round, small and protruding, milky white, opaque colonies with irregular edges (see figure 2 ). Under the light microscope, it is short rod-shaped, the spore is in the center, the cyst is not obviously enlarged, and the Gram stain is purple, which is a Gram-positive bacterium (see image 3 ).

[0044] (2) Physiological and biochemical properties of gjfn2

[0045] The physiological and biochemical identification of the bacterial strain gjfn2 mainly refers to the "Common Bacterial System Identification Manual" and "Berger's Bacterial Identification Manual". Bacillus ( Bacillus velezensis ) is consistent with the model strain.

[0046] Table 2 Determination results of physiological and biochemical characteristics of str...

Embodiment 3

[0052] Example 3 Bacillus Velez gjfn2 inhibits conidia germination and hyphal growth of pear rot fungus

[0053] (1) Inhibition of conidia germination of pear tree rot disease by Bacillus velez gjfn2

[0054] Preparation of Bacillus Velez gjfn2 fermentation broth: Inoculate the pure culture of Bacillus Velez gjfn2 into a 500 mL Erlenmeyer flask filled with 200 mL of NB culture solution, and culture at 160 r / min and 28°C for 1 ~2 days to bacterial solution OD 600 0.8 to 1.0.

[0055] Preparation of the filtrate of the fermentation broth of Bacillus veleisi gjfn2 (referred to as the fermentation filtrate): 200 mL of the fermentation broth was collected and centrifuged at 10,000 r / min for 20 min at 4°C, and the collected supernatant was filtered through a 0.22 µm microporous membrane Bacteria, stored at 4°C for later use.

[0056] Cut the pear tree bark tissue with the pycnidia of mature rotting bacteria and place it in 50 mL sterile water until the conidia horns fully overflo...

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Abstract

The present invention discloses bacillus velezensis and use thereof in preventing and treating diseases of bergamot pears and belongs to the technical field of biological prevention and treatment of plant diseases. A gjfn2 strain which is screened frombacteria of fruit and vegetable enzyme fermentation broth has a remarkable antibacterial effect on valsa mali var. pyri, erwinia amylovora and dickeya fangzhongdai, is identified as bacillus velezensis and preserved in China General Microbiological Culture Collection Center (CGMCC) on November 18, 2020 and has the preservation number of CGMCC No.21203. A bacillus velezensis gjfn2 fermentation broth and active metabolites in the fermentation broth have broad-spectrum resistance to the valsa mali var. pyri, erwinia amylovora and dickeya fangzhongdai, and have outstanding advantages of haivng high selectivity, being not prone to generating drug resistance, safe, efficient, environmentally friendly and the like, and having great potential inthe biological prevention and treatment of diseases of pear trees.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a strain of bacillus velei and its application in preventing and treating diseases of fragrant pears. Background technique [0002] China is one of the three origin centers of pear cultivation in the world, and it is also the main pear production area in the world. The pear planting area and output account for about 80% of the world's total pear cultivation area. Korla fragrant pear (referred to as fragrant pear) ( Pyrus sinkiangensi, Kuerlexiangli ) is a famous and special fruit in Xinjiang. It has been cultivated for 1,400 years and is mainly distributed in Korla and Aksu, Bazhou, Xinjiang. With the development of the fragrant pear industry, the expansion of the planting scale and the change of the cultivation mode, a variety of diseases are concurrent and difficult to control, which has become a prominent problem affecting production. Made f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
CPCC12N1/20A01N63/22C12R2001/07C12N1/205
Inventor 罗明郁帆韩剑盛强贺旭陈冰李燕
Owner XINJIANG AGRI UNIV
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