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Composite bacillus agent as well as preparation method and application thereof

A technology of bacillus and bacillus licheniformis, applied in the field of microorganisms, can solve the problems of large environmental impact and single action mechanism of a single strain, and achieve the effects of resisting pathogen infection, strengthening leaf protective film, and enhancing soil buffer capacity

Inactive Publication Date: 2021-02-05
HENAN BAIYU PLANT IMMUNE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the deficiencies in the above-mentioned prior art, the present invention provides a preparation method of a compound spore agent, the purpose is to solve the existing microbial source of pesticides or only a single strain can be applied to the prevention and control of one or a type of disease, but A single strain has a single mechanism of action and is greatly affected by the technical problems of the environment

Method used

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  • Composite bacillus agent as well as preparation method and application thereof
  • Composite bacillus agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of the composite spore agent provided in the present embodiment, the specific technical scheme is as follows:

[0021] The preparation method of compound spore bacterium agent, comprises the steps:

[0022] S1, Bacillus subtilis, Bacillus licheniformis, Bacillus natto, Bacillus megaterium, Bacillus coliformis and Bacillus amyloliquefaciens were respectively inoculated and activated in the activation medium; activation medium 10g peptone, 3g beef extract , 5g of sodium chloride, 15g of agar and 1000ml of sterile water were sterilized at 121°C for 30 minutes, the activation temperature was 20°C, and the activation time was 36h.

[0023] S2, put 20 parts of leftover rice residues and 5 parts of cassava residues through a pulverizer, add 2 parts of ferrous sulfate, 1 part of magnesium chloride, 0.5 parts of potassium dihydrogen phosphate and 1000 parts of sterile water, and sterilize them in an autoclave at 121 ° C. The first fermentation medium was ...

Embodiment 2

[0028] The preparation method of the composite spore agent provided in the present embodiment, the specific technical scheme is as follows:

[0029] The preparation method of compound spore bacterium agent, comprises the steps:

[0030] S1, Bacillus subtilis, Bacillus licheniformis, Bacillus natto, Bacillus megaterium, Bacillus coliformis and Bacillus amyloliquefaciens were respectively inoculated and activated in the activation medium; activation medium 10g peptone, 3g beef extract , 5g of sodium chloride, 15g of agar and 1000ml of sterile water were sterilized at 121°C for 30 minutes, the activation temperature was 35°C, and the activation time was 12h.

[0031] S2, put 30 parts of leftover rice residue and 10 parts of cassava residue through a pulverizer, add 4 parts of ferrous sulfate, 2 parts of magnesium chloride, 1 part of potassium dihydrogen phosphate and 1000 parts of sterile water, and sterilize in an autoclave at 121 ° C. The first fermentation medium was obtained...

Embodiment 3

[0035] The preparation method of the composite spore agent provided in the present embodiment, the specific technical scheme is as follows:

[0036] The preparation method of compound spore bacterium agent, comprises the steps:

[0037] S1, Bacillus subtilis, Bacillus licheniformis, Bacillus natto, Bacillus megaterium, Bacillus coliformis and Bacillus amyloliquefaciens were respectively inoculated and activated in the activation medium; activation medium 10g peptone, 3g beef extract , 5g of sodium chloride, 15g of agar and 1000ml of sterile water were sterilized at 121°C for 30 minutes, the activation temperature was 20°C, and the activation time was 24h.

[0038] S2, put 25 parts of leftover rice residues and 7 parts of cassava residues through a pulverizer, add 3 parts of ferrous sulfate, 1.5 parts of magnesium chloride, 0.8 parts of potassium dihydrogen phosphate and 1000 parts of sterile water and sterilize them in an autoclave at 121 ° C. The first fermentation medium wa...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a composite bacillus agent as well as a preparation method and application thereof. Various bacilli are mixed for secondary fermentation and are mixed with diatomite and fine-pored silica gel; the bacilli such as bacillus subtilis are effective microbial communities; different strains have the synergistic effect; after being applied to soil, the strains rapidly propagate to become dominant bacterial communities, and rhizosphere nutrition and resources of crops are controlled, so that pathogenic bacteriaof continuous cropping, root rot, rhizoctonia rot, gummosis, gray mold and the like lose living space and conditions, cell walls of plant root system cells are thickened, fibrillated and lignified, colloid double-silicon layers are formed, and invasion of the pathogenic bacteria is prevented; the diatomite and the fine-pored silica gel are mixed, so that the soil buffer capacity can be enhanced,water retention and heat preservation are realized, and the drought resistance and cold resistance of crops are enhanced; and meanwhile, leaf protective films can be strengthened, and pathogenic bacteria infection, disease resistance and insect resistance are achieved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound spore agent and its preparation method and application. Background technique [0002] Since the 21st century, the growth of population has led to a sharp increase in the demand for agricultural products. The abuse of chemical fertilizers and chemical pesticides is increasing day by day in order to increase the output of agricultural products. With the disadvantages of chemical pesticides and chemical fertilizers becoming increasingly prominent, some microbial-derived pesticides have gradually attracted great attention from scholars at home and abroad. The development and application of microbial-derived pesticides has gradually become a new trend in the pesticide industry. Its main advantages are: 1) a wide variety of products, with a large choice for research and development; 2) strong specificity, safe for non-target organisms, and less likely to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00A01P7/00C12R1/125C12R1/10C12R1/11C12R1/07
CPCC12N1/20A01N63/22
Inventor 柏玉兰王艳锦张强郭峰李鹏飞
Owner HENAN BAIYU PLANT IMMUNE TECH CO LTD
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