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Split type immunochromatographic detection device and immunochromatographic detection method

An immunochromatographic detection and split-type technology, which is applied in the detection field, can solve the problems of inconvenient use, many steps and consumables, etc., and achieve the effect of simplifying steps, improving sensitivity and accuracy

Pending Publication Date: 2021-02-02
SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above technical problems, the present invention proposes a split-type immunochromatography detection device and immunochromatography detection method, aiming to solve the problem that there are many steps and consumables in the sample pretreatment process of immunochromatography detection in the prior art, Inconvenient to use

Method used

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  • Split type immunochromatographic detection device and immunochromatographic detection method
  • Split type immunochromatographic detection device and immunochromatographic detection method
  • Split type immunochromatographic detection device and immunochromatographic detection method

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Embodiment 1

[0065] Sodium silicate (0.4 g / mL) ethanol aqueous solution was added into a 50 ml beaker, and ammonia water (0.1 mol / L) was slowly added dropwise. While adding ammonia water dropwise, soak the sample pad in the solution and wait for the reaction for 4 hours. After the reaction, the sample pad is taken out, dried at 90 degrees Celsius, and the dried sample pad is placed in the first shell of the first part to form the first part of the split-type immunochromatographic detection device for detecting malachite green.

Embodiment 2

[0067] Detection of malachite green in shrimp. Take out the split immunochromatography detection device of the present invention. Add 2 g of shrimp to a 15 ml centrifuge tube, add 3 ml of acetonitrile, vortex for 3 minutes, and centrifuge at 4000 rpm for 3 minutes to prepare an acetonitrile solution. Add 50 μL of acetonitrile solution dropwise to the first liquid addition part of the first part, let it stand for 3 minutes, and then repeat the dropwise addition 4 times. After drying, join the first part and the second part. Add 80 μL of chromatographic solution dropwise to the second liquid addition part of the second part, and observe the result after 3 minutes.

Embodiment 3

[0069] Determination of phorate in tea. Take out the split immunochromatography detection device of the present invention. Add 1 g of tea leaves to a 15 ml centrifuge tube, add 3 ml of 50% ethanol water, soak for 10 minutes, vortex for 3 minutes, and then centrifuge at 4000 rpm for 4 minutes to form a sample solution. Take 500 μL of sample solution and add it to the sample cup. Put the sample cup into the heater, put the first part into the sample cup, and control the heater to heat at 70 degrees for 10 minutes. Take the first part and insert it into the second part, binding in place. Add 80 μL of 0.1M pH7 phosphate-buffered saline (PBS) to the second liquid addition part of the second part for chromatography, and observe the results through the observation window after 5 minutes.

[0070] Beneficial effects of the present invention: by separating the sample pad of the immunochromatography detection device for absorbing the sample liquid from the test strip, as an independe...

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Abstract

The invention provides a split type immunochromatography detection device and an immunochromatography detection method. The split type immunochromatography detection device comprises a first part anda second part. The first part comprises a first shell and a sample pad which is arranged in the first shell, one part of the sample pad is exposed out of the first shell, the first shell is provided with a first liquid adding part, and the first liquid adding part is at least used for adding chromatographic liquid. The second part comprises a second shell and a test strip arranged in the second shell. The first part is independent of the second part and can be detachably connected with the second part; when the first part is connected with the second part, the split type immunochromatography detection device can absorb chromatography liquid through the first liquid adding part, and the sample pad is in contact with the test strip. According to the device and the method disclosed in the scheme, the functions of the sample pad are utilized to the maximum extent, the steps of the sample pretreatment process and used consumables are simplified, and the sensitivity and accuracy of immunochromatographic detection are improved.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a split-type immunochromatography detection device and an immunochromatography detection method. Background technique [0002] Existing immunochromatographic detection devices generally include a bottom plate, on which a sample pad, a chromatographic membrane, and a water-absorbing pad are sequentially overlapped. Immunochromatography detection generally includes sample pretreatment process and chromatography process. Generally, the sample is pretreated, and the target substance is converted into a test solution (including sample solution and chromatography solution) by various means, and then detected by immunochromatography. The sample pad at the front end of the device absorbs liquid and guides it to the chromatographic membrane for immune recognition. Therefore, the sample pad only plays a drainage role in the current immunochromatographic detection device. When needed, so...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/54306G01N33/558
Inventor 严义勇马红圳邓炀蔡振庆
Owner SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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