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A kind of qualitative and quantitative detection method of aconitine phytotoxin

A quantitative detection method, aconitine technology, is applied in the field of qualitative and quantitative detection of aconitine-like phytotoxins, which can solve the problems of low detection limit and achieve the effects of rapid response, low detection cost and high detection sensitivity

Active Publication Date: 2022-07-05
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY +1
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  • Abstract
  • Description
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Problems solved by technology

The relevant research on the detection of alkaloid phytotoxins by SERS technology disclosed in the prior art mainly focuses on isoquinoline derivatives and tropane derivatives, and the detection limit is low

Method used

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  • A kind of qualitative and quantitative detection method of aconitine phytotoxin
  • A kind of qualitative and quantitative detection method of aconitine phytotoxin
  • A kind of qualitative and quantitative detection method of aconitine phytotoxin

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Embodiment 1

[0032] like figure 1 As shown, a qualitative and quantitative detection method for aconitine phytotoxins comprises the following steps:

[0033] (1) In a 96-well plate, mix 5-100 μg of 200 μL of aconitine, aconitine, hypoconitine, and mixed aconitine (mixture of aconitine, mesaconitrile, hypoaconitinine) / L of the standard solution with a concentration of 1.5 × 10 in 50 μL -8 The silver nanoparticle sol of M is mixed evenly, and then 50 μL of KI solution with a concentration of 1M is added to obtain the standard solution to be tested; the particle size of the silver nanoparticle sol is 100 nm;

[0034] (2) Place the above 96 wells under the microscope, adjust the microscope so that the laser of the Raman spectrometer is focused on the liquid surface into a bright circular light plate for detection, and scan the dark current before the test to reduce the interference of the background, using a wavelength of 785nm laser, the integration time is 2s, the accumulation times are 5...

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Abstract

The invention discloses a qualitative and quantitative detection method for aconitine-type phytotoxins, which comprises the following steps: (1) standard solutions of aconitine, mesoconitine, hypoaconitine and mixed aconitine with different concentrations are prepared Respectively mix with silver nanoparticle sol uniformly, then add the solution of inorganic salt to obtain the standard liquid to be tested; (2) use Raman spectrometer to detect the above-mentioned standard liquid to be tested, and record the position and intensity of characteristic peaks; (3) to test the liquid to be tested; The sample obtains the sample liquid to be tested according to the same method as in step (1), uses a Raman spectrometer to detect the sample liquid to be tested, records the position and intensity of the characteristic peak, and according to step (2), the aconitine plants in the sample to be tested are The types of toxins were qualitatively and quantitatively analyzed. The invention has the advantages of simple and rapid detection process, rapid response, low detection cost and high detection sensitivity, and can meet the actual demand for on-site detection of plant toxins.

Description

technical field [0001] The invention belongs to the technical field of Raman detection, in particular to a qualitative and quantitative detection method of aconitine phytotoxins. Background technique [0002] Aconitine is a class of highly toxic phytotoxins widely existing in the environment. After entering the human body through food, skin contact, etc., it will cause serious poisoning reactions and even life-threatening. The commonly used detection methods of aconitine phytotoxins are high performance liquid chromatography, liquid chromatography tandem mass spectrometry and gas chromatography-mass spectrometry, etc. However, these methods require expensive equipment, complicated operation and long detection time, which are difficult to meet the needs of The practical needs of on-line monitoring and on-site emergency detection limit their wide application. Therefore, the development of cost-effective and rapid and sensitive detection methods for aconitine-like phytotoxins ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N1/28
CPCG01N21/65G01N1/28
Inventor 刘国坤王维礼卢江龙顾家镭田中群
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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