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A method of colchicine-induced polyploidy in Phalaenopsis protocorm

A technology of colchicine and protocorm, applied in the field of polyploid breeding, can solve the problems of low induction rate, low survival rate of protocorm, etc., and achieve the effect of reducing the number of stomata

Active Publication Date: 2022-07-12
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI +1
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Problems solved by technology

[0004] The difficulty of solving the above problems and defects is: the concentration and treatment time of the colchicine co-cultivation method to induce protocorms need to be moderate, too high will make the survival rate of protocorms too low, and too low will make the induction rate too low

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  • A method of colchicine-induced polyploidy in Phalaenopsis protocorm
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[0051] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0052] In view of the problems existing in the prior art, the present invention provides a method for inducing polyploidy from protocorm of Phalaenopsis by colchicine. The present invention is described in detail below with reference to the accompanying drawings.

[0053] like figure 1 As shown, the method for colchicine-induced polyploidy in Phalaenopsis protocorm provided by the embodiment of the present invention comprises the following steps:

[0054] S101, polyploidy induction: transfer protocorm to 1 / 2MS+1mg / l6-BA+0.2mg / l NAA+5g / l agar+20g / l sucrose solid medium containing colchicine for induction treatment ...

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Abstract

The invention belongs to the technical field of polyploid breeding, and discloses a method for inducing polyploidy from protocorm of Phalaenopsis phalaenopsis by colchicine, comprising: transferring the protocorm to 1 / 2MS+1 mg containing 0.05% colchicine / l 6-BA+0.2mg / l NAA+5g / l agar+20g / l sucrose solid medium for 15 days to induce induction treatment; rinse with sterile water 3-4 times after treatment, and use filter paper to absorb moisture, Transferred to 1 / 2MS+5g / l agar+20g / l sucrose medium, subcultured 2-3 times; polyploid identification: morphological observation, stomatal identification and ploidy identification were performed respectively; data statistics. In the invention, different concentrations of colchicine and different treatment times are used to double the co-cultivation method for the protocorm of Phalaenopsis, which lays a foundation for the polyploid induction and cultivation of new Phalaenopsis varieties with large flowers, bright colors and high ornamental value.

Description

technical field [0001] The invention belongs to the technical field of polyploid breeding, and in particular relates to a method for inducing polyploidy from protocorm of Phalaenopsis by colchicine. Background technique [0002] At present, Phalaenopsis is a plant of the Orchidaceae Phalaenopsis, with a wide variety of flowers and shapes resembling butterflies. In the flower market, Phalaenopsis is very popular in the flower market for its large and colorful flowers. The cultivated species are mainly tetraploid, triploid and aneuploid, of which tetraploid accounts for the vast majority, up to 82%. In addition, polyploidy often has the advantages of stout plant type, huge leaf and flower organs, and strong stress resistance. Therefore, polyploidy breeding induction of Phalaenopsis is an indispensable method for Phalaenopsis breeding. Orchid tissue culture technology is relatively mature, so most of orchids use tissue culture combined with doubling for polyploid induction, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 葛红吴婷贾瑞冬杨树华朱剑非赵鑫李秋香
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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