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Composition for cell transplant, and method for cell transplant

A technology of cell transplantation and composition, applied in biochemical equipment and methods, drug combinations, animal cells, etc., can solve problems such as mixing, and achieve the effect of improving persistence and proliferation

Pending Publication Date: 2021-01-29
KYOTO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the material for cell transplantation therapy described in Patent Document 1, an animal-derived material such as gelatin is used as the hydrogel, so cells that may cause infection or cause an immune response may be mixed into the transplant site. Allergen substances or other impurities of animal origin, etc.

Method used

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  • Composition for cell transplant, and method for cell transplant
  • Composition for cell transplant, and method for cell transplant

Examples

Experimental program
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Effect test

manufacture example 1

[0140] ο Production of SELP8K

[0141] According to the method described in the Examples of Japanese Patent No. 4088341, plasmid pPT0345 encoding SELP8K was prepared.

[0142] The prepared plasmid was transformed into Escherichia coli to obtain a SELP8K producing strain.

[0143] Using the overnight culture solution of the SELP8K producer strain grown at 30°C, it was inoculated in 50 ml of LB medium in a 250 ml flask. Kanamycin was added to a final concentration of 50 µg / ml, and the culture solution was cultured while stirring (200 rpm) at 30°C. When the culture solution reached OD600=0.8 (using an absorbance meter UV1700: manufactured by Shimadzu Corporation), 40 ml was transferred to a flask previously warmed to 42° C., and incubated at the same temperature for about 2 hours. The culture was cooled on ice, and the OD600 of the culture solution was measured. E. coli were collected by centrifugation. In order to extract protein from the collected Escherichia coli, ultrason...

manufacture example 2

[0154] In Production Example 1, a protein (A-2) having a molecular weight of about 82 kDa sequence (16) was similarly obtained except that "plasmid pPT0364 encoding SELPOK" was used instead of "plasmid pPT0345 encoding SELP8K".

manufacture example 3

[0156] In Production Example 1, a protein (A-3) having a molecular weight of about 93 kDa sequence (17) was obtained in the same manner except that "pSY1398-1 encoding SLP4.1" was used instead of "plasmid pPT0345 encoding SELP8K".

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Abstract

Provided are a composition for cell transplant and a method for cell transplant that allow myocytes and / or cardiac progenitor cells to be suitably maintained in myocardial tissue and allow the survival and proliferation of transplanted cells to be improved. This composition for cell transplant comprises cells and an aqueous solution of a protein (A), and is characterized in that: the cells are myocardial cells and / or cardiac progenitor cells; protein (A) has a hydrophobicity of 0.2-1.2; the protein (A) has a polypeptide chain (Y) and / or a polypeptide chain (Y'); the total number of polypeptidechains (Y) and polypeptide chains (Y') in the protein (A) is 1-100; the polypeptide chain (Y) is an amino acid sequence (X) repeated 2-100 times, the amino acid sequence being any one of a VPGVG sequence (1) which is an amino acid sequence represented by SEQ ID NO:1, a GVGVP sequence (2) which is an amino acid sequence represented by SEQ ID NO:2, a GPP sequence, a GAP sequence, and a GAHGPAGPK sequence (3), which is an amino acid sequence represented by SEQ ID NO:3; the polypeptide chain (Y') is the polypeptide chain (Y) where 0.1-5% of the amino acid residues thereof are substituted with lysine residues and / or arginine residues; and the total number of the lysine residues and arginine residues is 1-100.

Description

technical field [0001] The present invention relates to a composition for cell transplantation and a cell transplantation method. Background technique [0002] Regenerative medicine is attracting attention as a treatment for heart failure such as myocardial infarction. The purpose of regenerative medicine is to transplant cells, etc. into organs or tissues that have been damaged by injury or disease, and to restore the function of the damaged tissues or organs, which may become difficult to treat at present. Potential possibility of the only treatment for cases of technology. Adult cardiomyocytes are extremely lack of self-replication ability, and it is very difficult to repair the myocardial tissue when it is damaged. In order to repair damaged myocardial tissue, an attempt has been made to transplant a graft or a myocardial sheet containing cardiomyocytes produced by a cell engineering method into an affected area (Patent Document 1). However, this method has a problem ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/22A61L27/38A61P9/00A61P9/10A61P29/00A61K35/34A61K35/545A61K38/16A61K38/39
CPCA61K35/545A61K35/34C12N5/0657A61L27/3826A61L27/3882A61L27/227A61L2430/20A61L2300/252A61L2400/06A61P9/10C12N2501/998A61K2300/00A61K38/164
Inventor 吉田善纪羽溪健铃木凉介川端慎吾
Owner KYOTO UNIV
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