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Preparation method and application of microneedle electrode

An electrode and microneedle technology, which is applied in the field of preparation of microneedle electrodes, can solve problems such as limiting the analytical sensitivity of electrochemical biosensors, achieve good electrocatalytic properties and improve detection sensitivity.

Active Publication Date: 2021-01-22
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a preparation method and application of a microneedle electrode, to solve the problem of low electroactive nanomaterials loaded on needle-shaped platinum wire microelectrodes, Issues Limiting the Analytical Sensitivity of the Resulting Electrochemical Biosensors

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  • Preparation method and application of microneedle electrode
  • Preparation method and application of microneedle electrode
  • Preparation method and application of microneedle electrode

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Synthesis of platinum wire microneedle electrodes and silver / silver chloride wire microneedle electrodes:

[0032] Melt platinum with a purity of 99.999% at high temperature to obtain liquid platinum, and then carry out the process of pressing square strips → wire drawing → shaping → grinding to obtain such figure 1 The lower diameter shown is a 200 μm platinum wire microneedle electrode. In the same way, 99.999% pure silver is melted at high temperature to obtain liquid silver, and then the process of pressing square bar → wire drawing → shaping → grinding process is obtained as follows: figure 1 The silver wire microneedle shown is followed by the silver wire microneedle in 1mol / L potassium chloride solution, under the voltage of -0.1V, the constant potential deposition is 1000s, and the silver / silver chloride electrode is obtained as the reference electrode .

[0033] (2) Synthesis of polydopamine spheres-gold nanocomposites:

[0034] Dopamine was dissolved in...

Embodiment example 2

[0040] (1) Synthesis of platinum wire microneedle electrodes and silver / silver chloride wire microneedle electrodes:

[0041] Melt platinum with a purity of 99.999% at high temperature to obtain liquid platinum, and then carry out the process of pressing square strips → wire drawing → shaping → grinding to obtain such figure 1 A platinum wire microneedle electrode with a diameter of 200 μm is shown. In the same way, 99.999% pure silver is melted at high temperature to obtain liquid silver, and then the process of pressing square bar → wire drawing → shaping → grinding process is obtained as follows: figure 1 The silver wire microneedle shown is followed by the silver wire microneedle in 1mol / L potassium chloride solution, under the voltage of -0.1V, the constant potential deposition is 1000s, and the silver / silver chloride electrode is obtained as the reference electrode .

[0042] (2) Synthesis of polydopamine spheres-gold nanocomposites:

[0043] Dopamine was dissolved in d...

Embodiment example 3

[0049] (1) Synthesis of platinum wire microneedle electrodes and silver / silver chloride wire microneedle electrodes:

[0050] Melt platinum with a purity of 99.999% at high temperature to obtain liquid platinum, and then carry out the process of pressing square strips → wire drawing → shaping → grinding to obtain such figure 1 A platinum wire microneedle electrode with a diameter of 50 μm under the structure shown. In the same way, 99.999% pure silver is melted at high temperature to obtain liquid silver, and then the process of pressing square bar → wire drawing → shaping → grinding process is obtained as follows: figure 1 The silver wire microneedle shown is followed by the silver wire microneedle in 1mol / L potassium chloride solution, under the voltage of -0.1V, the constant potential deposition is 1000s, and the silver / silver chloride electrode is obtained as the reference electrode .

[0051] (2) Synthesis of polydopamine spheres-gold nanocomposites:

[0052] Dopamine ...

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Abstract

The invention discloses a preparation method and application of a microneedle electrode. The preparation method comprises the following steps: preparing a silver wire microneedle electrode and a platinum wire microneedle electrode; loading a layer of silver chloride on the surface of the silver wire microneedle electrode in a potassium chloride solution through constant potential electrodepositionto obtain a silver-silver chloride microneedle electrode; preparing a dopamine aqueous solution and an ammonia aqueous solution, and preparing polydopamine spheres; preparing a noble metal saline solution, and adding the polydopamine spheres into the cooled noble metal saline solution to prepare a polydopamine sphere-noble metal composite material; modifying the platinum wire microneedle electrode with the polydopamine sphere-noble metal composite material, and applying the product to cell and tumor tissue detection. In-vivo detection of the electrode can be effectively achieved, the total amount of Au participating in electrode reaction is increased through polydopamine, gold nanoparticles have good electrocatalytic properties, and the electrode detection sensitivity can be improved through the synergistic effect of the gold nanoparticles and the polydopamine.

Description

technical field [0001] The invention relates to the technical field of electrode preparation, in particular to a method for preparing a microneedle electrode and its application. Background technique [0002] Miniaturization is one of the development trends of analytical chemistry. In the field of electrochemical analysis, it is mainly reflected in the miniaturization of working electrodes. Such products have the ability to realize real-time monitoring of chemical information in the body and improve its stability. As the frontier field of electroanalytical chemistry, microelectrode technology provides a powerful means for people to conduct ultra-sensitive detection at the cell level, thereby exploring cell physiological phenomena and revealing the laws of life activities. Compared with conventional electrodes and millimeter electrodes, when the one-dimensional size of the electrode is reduced to the micron or nanometer level, the electrochemical reaction on the electrode wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 王琳王征张艳李琪琳
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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