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Stable vaginal secretion visible component staining solution and preparation method thereof

A technology of vaginal secretions and dyeing solution, applied in the field of dyeing solution, can solve the problems of cumbersome dyeing steps, small number of daily readings, poor use effect, etc., and achieve the effect of clear distinction, excellent cell shape and high accuracy

Pending Publication Date: 2021-01-12
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to solve the problems of cumbersome dyeing steps, complex operation, slow dyeing speed, poor use effect, high cost, and small daily reading volume in the prior art, and provide a stable dyeing method for the formed components of vaginal secretions. Liquid and its preparation method

Method used

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  • Stable vaginal secretion visible component staining solution and preparation method thereof
  • Stable vaginal secretion visible component staining solution and preparation method thereof
  • Stable vaginal secretion visible component staining solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Stable vaginal discharge formed component staining solution, including staining solution A and staining solution B, the formula is as follows:

[0071]

[0072]

[0073] The preparation method of the above-mentioned stable vaginal secretion formed component staining solution:

[0074] ① Preparation before weighing:

[0075] Select the equipment and glassware with suitable measurement accuracy, such as the choice of electronic balance, electronic scale, measuring cylinder, stainless steel medicine spoon, and confirm the equipment and utensils used before weighing.

[0076] ② Weighing and dissolving: Staining solution B weighs the chemicals in turn according to the preparation process. After each chemical is completely dissolved, add the next chemical, and use a magnetic stirrer or overhead stirrer to stir and dissolve. After all the chemicals are dissolved, continue to dissolve. Stir for 20 minutes, adjust the pH to 6.50±0.50 (25±1°C) with acetic acid or 1M sodium...

Embodiment 2

[0087] Stable vaginal discharge formed component staining solution, including staining solution A and staining solution B, the formula is as follows:

[0088]

[0089] The preparation method of the above-mentioned stable vaginal secretion formed component staining solution:

[0090] ① Preparation before weighing:

[0091] Select the equipment and glassware with suitable measurement accuracy, such as the choice of electronic balance, electronic scale, measuring cylinder, stainless steel medicine spoon, and confirm the equipment and utensils used before weighing.

[0092] ② Weighing and dissolving: Staining solution B weighs the chemicals in turn according to the preparation process. After each chemical is completely dissolved, add the next chemical, and use a magnetic stirrer or overhead stirrer to stir and dissolve. After all the chemicals are dissolved, continue to dissolve. Stir for 20 minutes, adjust the pH to 6.50±0.50 (25±1°C) with acetic acid or 1M sodium hydroxide; ...

Embodiment 3

[0103] Stable vaginal discharge formed component staining solution, including staining solution A and staining solution B, the formula is as follows:

[0104]

[0105] The preparation method of the above-mentioned stable vaginal secretion formed component staining solution:

[0106] ① Preparation before weighing:

[0107] Select the equipment and glassware with suitable measurement accuracy, such as the choice of electronic balance, electronic scale, measuring cylinder, stainless steel medicine spoon, and confirm the equipment and utensils used before weighing.

[0108] ② Weighing and dissolving: Staining solution B weighs the chemicals in turn according to the preparation process. After each chemical is completely dissolved, add the next chemical, and use a magnetic stirrer or overhead stirrer to stir and dissolve. After all the chemicals are dissolved, continue to dissolve. Stir for 20 minutes, adjust the pH to 6.50±0.50 (25±1°C) with acetic acid or 1M sodium hydroxide; ...

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Abstract

The invention relates to a stable vaginal secretion visible component staining solution and a preparation method thereof, and belongs to the technical field of staining solutions. The problems that inthe prior art, dyeing steps are tedious, operation is complex, the dyeing speed is low, the using effect is poor, the cost is high, and the daily film reading amount is small are solved. The stable vaginal secretion visible component staining solution comprises a staining solution A and a staining solution B, wherein the staining solution A comprises an organic solvent and a staining agent, and the staining agent is a red system staining agent, a green system staining agent, a purple system staining agent or a blue system staining agent; and the staining solution B comprises a buffer solution, a preservative, a dyeing assistant, an osmotic pressure regulator and a surfactant. The staining solution is excellent in detected cell morphology, high in accuracy and good in stability, and the validity period can reach 12 months.

Description

technical field [0001] The invention belongs to the technical field of dyeing solution, and in particular relates to a stable dyeing solution of formed components of vaginal secretions and a preparation method thereof. Background technique [0002] For vaginal secretions, in medical clinical testing, the most commonly used method is optical microscopy. Before testing, the collected original samples must be filtered, diluted and mixed to make the samples meet the requirements of microscope testing. Then, the samples need to be Prepare glass slide samples suitable for microscopic examination, and the samples are divided into "dry slides" and "wet slides". Wet slide preparation methods mainly include counting cell method and coverslip method. The counting cell method is to fill the sample solution (stained or not) into the optical counting cell, and then through natural or artificial sedimentation to fix the microorganisms (cells) in an observable position, and then send the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 朱子是孙德亚林月赵亚荣王嘉星高威何浩会
Owner DIRUI MEDICAL TECH CO LTD
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