Preparation method of hypoallergenic vegetable protein meat
A plant protein and protein technology, which is applied in the field of preparation of hypoallergenic plant protein meat, can solve the problems of energy consumption, low efficiency, and unfriendly environment, and achieve the goal of reducing allergens, improving the quality of plant protein, and specifically degrading Effect
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Embodiment 1
[0044] Example 1 Degradation of Allergens in Plant Tissue Protein by Biological Enzyme
[0045] Enzymatic desensitization is to reduce the effect of protein sensitization by changing the degree of enzymatic hydrolysis of protease or protein structure. If the degree of hydrolysis is appropriate, the structure of the allergen epitope can be changed to reduce the allergenicity; if the degree of hydrolysis is not appropriate, the internal allergen epitope can be exposed to increase the allergenicity; if the degree of hydrolysis is deep, the protein can be hydrolyzed into Small peptides or amino acids reduce their allergenicity, but some functional properties of proteins may be deteriorated. Enzymatic cross-linking will form covalent bonds between protein molecules or between internal polypeptide chains, leading to protein aggregation, and embedding the antigenic epitopes exposed on the surface into the interior of protein molecules, thereby eliminating its allergenicity; but betwe...
Embodiment 2
[0049] Example 2 Degradation of Allergens in Plant Tissue Protein by Microbial Method
[0050] The selected strains of Bacillus subtilis, Lactobacillus plantarum, Saccharomyces cerevisiae, and Aspergillus oryzae have significant differences in the degradation effects of soybean allergens when fermenting soybean protein powder under liquid fermentation and solid state fermentation.
[0051] Using Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae to ferment soybean protein, the results show that the content of Glym series allergens in soybean protein is reduced by 75%. Using Bacillus subtilis and Aspergillus oryzae to ferment soybean protein, the content of Glym series allergens in soybean protein is reduced by 80%.
[0052] Through single factor experiment and response surface design, it is determined that the suitable fermentation conditions for the degradation of total soybean allergens by Lactobacillus plantarum liquid fermented soybean meal powder are ...
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