A kind of preparation method of gold-silver nanocluster protected by glutathione thiotransferase and its application in the detection of oxytetracycline
A technology of glutathione and silver nanoclusters, applied in chemical instruments and methods, measuring devices, material excitation analysis, etc., can solve the problems of accumulation, water and soil pollution, low selectivity, etc., and achieve good detection sensitivity, low toxicity, Quick and easy results
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Embodiment 1
[0040] Example 1: Optimization of the ratio of gold ions to silver ions; 120 μL of 10 mM chloroauric acid aqueous solution, 500 μL of 10.0 mg / ml glutathione transferase aqueous solution and 320 μL of ultrapure aqueous solution were mixed and stirred. After 10 s, adjust the pH to 11 by adding 40 μL of 1 M NaOH solution. The solution changed from light yellow to colorless within 1 minute, then 30 μL of 10 mM silver nitrate aqueous solution was added, rapidly heated to 70° C., and reacted for 1 hour.
Embodiment 2
[0041]Example 2: Optimization of the ratio of gold ions to silver ions; 120 μL of 10 mM chloroauric acid aqueous solution, 500 μL of 10.0 mg / ml glutathione transferase aqueous solution and 320 μL of ultrapure aqueous solution were mixed and stirred. After 10 s, adjust the pH to 11 by adding 40 μL of 1 M NaOH solution. The solution changed from light yellow to colorless within 1 minute, and then 20 μL of 10 mM silver nitrate aqueous solution was added, rapidly heated to 70° C., and reacted for 1 hour.
Embodiment 3
[0042] Example 3: Optimization of the ratio of gold ions to silver ions; 120 μL of 10 mM chloroauric acid aqueous solution, 500 μL of 10.0 mg / ml glutathione transferase aqueous solution and 320 μL of ultrapure aqueous solution were mixed and stirred. After 10 s, adjust the pH to 11 by adding 40 μL of 1 M NaOH solution. The solution changed from light yellow to colorless within 1 minute, then 15 μL of 10 mM silver nitrate aqueous solution was added, rapidly heated to 70° C., and reacted for 1 hour.
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