DHA enriched polyunsaturated fatty acid compositions
A lipid composition, the technology of the composition, applied in the directions of drug combination, edible oil/fat, anhydride/acid/halide active ingredient, etc.
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example 1
[0120] Example 1 - Extraction of DHA canola oil from seeds
[0121] Various canola species disclosed in U.S. Patent Publication No. US 2018 / 0016590 A1 are grown as summer crops. Seeds were harvested and then stored at room temperature prior to extrusion.
[0122] 272 kg of seeds were pressed using a Kern Kraft KK80 screw press to produce DHA oil. Set the press collar heater temperature to the maximum setting on the thermostat. The initial ambient temperature and choke coil temperature were 20° C., and the choke distance was set to 73.92 mm. Feed the seeds and continue to collect oil and meal until all the seeds have been squeezed before stopping the oil press.
[0123] The rotational speed of the auger, the temperature of the meal and the discharged oil are monitored throughout the pressing process. The extrusion time for 270 kg is 4 hours, which is a throughput rate of 67.5 kg / hour. A yield of 87.2 kg (32%) of crude oil was obtained. After filtration to remove fines, ...
example 2
[0124] Example 2 - Reference Oil Blend
[0125] Pure fish oil contains low levels of ALA fatty acids and significantly higher levels of EPA and DHA. A reference oil blend (referred to herein as "crude triglyceride reference blend oil" or similar) was designed to be as similar in composition as possible to the filtered DHA canola oil obtained in Example 1. This was done by (a) matching the total amount of DHA to the total amount of DHA in canola oil, and (b) matching the ratio of DHA / (ALA+EPA). This is accomplished by blending DHA-enriched fish oil (tuna), ALA-enriched oil (linseed oil), and standard canola oil. The resulting reference blend also had a similar total omega-3 content to DHA canola oil.
example 3
[0126] Example 3 - Fatty acid composition of crude DHA canola oil and reference blend
[0127] Analyze the fatty acid composition of filtered crude and reference blend oils. The result is shown below.
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