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Extraction method and application of cistanche polysaccharide

A technology of Cistanche deserticola polysaccharide and extraction method, which is applied in the field of extraction of Cistanche deserticola polysaccharide and can solve the problem of not being able to effectively treat acute pancreatitis and the like

Active Publication Date: 2020-12-29
GUANGZHOU YUMING BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, there is no clinical drug that can effectively treat acute pancreatitis (AP). The treatment of AP is mainly supportive, that is, in the case of other organs being involved, analgesics and related measures are used for supportive care

Method used

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  • Extraction method and application of cistanche polysaccharide
  • Extraction method and application of cistanche polysaccharide
  • Extraction method and application of cistanche polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of embodiment 1 Cistanche deserticola polysaccharide

[0042] 1. Experimental materials

[0043] Desert cistanche.

[0044] 2. Experimental enzymes

[0045] Enzymes used to extract polysaccharides from Cistanche deserticola in the experiment of table 1

[0046]

[0047] 3. Experimental equipment

[0048] Table 2 The experimental equipment used to extract polysaccharides from Cistanche deserticola

[0049]

[0050] 4. Cistanche herbal degreasing pretreatment

[0051] The degreasing treatment method of Cistanche medicinal materials is as follows:

[0052] (1) Dried the decoction pieces of Cistanche deserticola at 60°C, cooled naturally, softened when heated and dried, and hardened when cooled, then pulverized and passed through a 10-mesh sieve to obtain coarse powder of Cistanche deserticola;

[0053] (2) Add 6400ml, 6400ml and 4800ml of ethyl acetate to 800g of cistanche coarse powder to extract three times, each time for 2 hours, filte...

Embodiment 2

[0130] Example 2 Pharmacodynamic experiment of Cistanche deserticola polysaccharides of the present invention for preventing or treating acute pancreatitis (AP)

[0131] 1 experimental equipment

[0132]Electronic analytical balance, Shanghai Youke Instrument Co., Ltd., model FA2204B. Balance, Changzhou Keyuan Electronic Instrument Co., Ltd., model JA30002. Vortex mixer, Haimen Qilinbeier Instrument Manufacturing Co., Ltd., model GL-88B. Flight board control box, Wuhan Yihong Technology Co., Ltd., model DFPCO2B. Drosophila anesthesia blow tube, Wuhan Yihong Technology Co., Ltd., model YHDAG2. Stereo microscope, Phoenix Optical Co., Ltd., model xk-3. Gas cylinder cabinet, Gansu Zhicheng Science and Education Equipment Co., Ltd., model 500*450*1900. Biochemical incubator, Shanghai Jinghong Experimental Equipment Co., Ltd., model SHP-250. Drosophila tubes, Haimen Zhongtai Experimental Equipment Factory, model 24*95mm.

[0133] 2 experimental reagents

[0134] Acetaldehyde...

Embodiment 3

[0220] The extraction method of Cistanche deserticola polysaccharide of the present invention is as follows:

[0221] (1) Dried the decoction pieces of Cistanche deserticola at 60°C, cooled naturally, softened when heated and dried, and hardened when cooled, then pulverized and passed through a 10-mesh sieve to obtain coarse powder of Cistanche deserticola;

[0222] (2) Add 6400ml, 6400ml and 4800ml of ethyl acetate to 800g of cistanche coarse powder to extract three times, each time for 2 hours, filter and collect medicinal material residue;

[0223] (3) Add 6400ml, 6400ml and 4800ml of 95% ethanol successively to the medicinal material residue in step (2) to extract three times, each time for 2 hours, filter, collect medicinal material residue, and obtain Cistanche deserticola powder after degreasing;

[0224] (5) Add papain (2%), cellulase (2%), and pectinase (0.4%) to the degreased Cistanche powder, add water to make the ratio of solid to liquid 1:20 times (extract in 3 ti...

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Abstract

The invention provides a cistanche deserticola polysaccharide extraction method which comprises the following steps: (1) extracting cistanche deserticola medicinal material coarse powder with ethyl acetate and ethanol in sequence, and collecting medicine residues to obtain degreased cistanche deserticola powder; (2) adding papain, cellulase and pectinase into degreased cistanche deserticola powder, adding water to enable the solid-liquid ratio to be 1: 20-1: 25 times, performing enzymolysis, performing ultrasonic extraction for 0.5-0.75 hour for 1-3 times, combining filtrates, centrifuging, taking a supernatant, concentrating, adding ethanol into a concentrated solution until the total concentration of the ethanol is 80 percent, and standing; centrifuging to obtain precipitate, and freeze-drying the precipitate. The invention further provides application of the cistanche polysaccharide in preparation of a medicine for preventing or treating acute alcoholic pancreatitis. The cistanche deserticola polysaccharide is extracted by using the method.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from Cistanche deserticola and its application. Background technique [0002] The scientific name of Cistanche deserticola Ma, also known as Cistanche deserticola and Cunyun, is mainly distributed in Xinjiang, Gansu and Ningxia of my country, and is an endangered species. Cistanche has always been known as "desert ginseng". Cistanche, as a traditional Chinese herbal medicine, has high medicinal value and is frequently used in prescriptions for nourishing the kidney and strengthening yang in ancient my country. [0003] According to Deng Nan et al.’s description in the research progress of the pharmacological effects of Cistanche polysaccharides: Cistanche is rich in chemical components, including phenylethanol glycosides, iridoid glycosides, polysaccharides, alkaloids, lignans and some Volatile components, the active component polysaccharides are mainly composed of glucose, rhamnose, ara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/18
CPCC08B37/0003A61K31/715A61P1/18
Inventor 刘小花陈新平程世红辛振辉
Owner GUANGZHOU YUMING BIOLOGICAL TECH CO LTD
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