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IgG, IgM and IgA antibody combined detection kit for 2019-nCoV and detection method thereof

A 2019-ncov and kit technology, which is applied in the field of biomedicine, can solve problems such as qualitative and quantitative judgment, and affect the accuracy of detection results of 2019-nCoV infection, so as to avoid false negatives, reduce biosafety risks, and improve detection efficiency Effect

Active Publication Date: 2020-12-22
深圳爱信生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the fact that the existing 2019-nCoV antibody detection kits or other products cannot simultaneously accurately determine the IgG, IgM and IgA antibody subtypes of 2019-nCoV at the same time, which will affect the accuracy of the detection results of 2019-nCoV infection Problem, the present invention provides a combination detection kit for IgG, IgM and IgA antibodies of 2019-nCoV and its detection method

Method used

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  • IgG, IgM and IgA antibody combined detection kit for 2019-nCoV and detection method thereof
  • IgG, IgM and IgA antibody combined detection kit for 2019-nCoV and detection method thereof

Examples

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Effect test

Embodiment 1

[0056] A combined detection kit for 2019-nCoV IgG, IgM and IgA antibodies, including 2019-nCoV marker protein coated plate, sample diluent, washing solution, anti-human IgA antibody enzyme labeling solution, anti-human IgG antibody enzyme labeling work solution, anti-human IgM antibody enzyme-labeled working solution, chromogenic substrate, stop solution and positive control; the positive control is the complex of anti-2019-nCoV marker protein monoclonal antibody IgG-human IgA / IgG / IgM antibody.

[0057] Among them, the preparation method of the 2019-nCoV protein-coated plate is:

[0058] a. Antigen protein coating: Dilute 2019-nCoVS1 protein and 2019-nCoV N protein with coating solution (0.01M sodium phosphate solution, pH 7.4) to 1 μg / mL, then mix according to 1:1 volume ratio and add Into a polystyrene 96-well plate with high binding capacity, add 0.1 mL per well, seal the sealing film, and place in a 37°C thermostat for 2 hours;

[0059] b. Plate washing: Shake off the exc...

Embodiment 2

[0072] Using the kit in Example 1 to detect 11 inactivated 2019-nCoV antibody positive sera and 31 inactivated 2019-nCoV antibody negative sera, the specific detection method includes the following steps:

[0073] S1. Primary antibody incubation: Mix 11 positive sera and 31 negative sera to be tested with the sample diluent at a volume of 1:100 to obtain 42 diluted samples. Take 0.1 mL of the above 42 diluted samples and add them to the In the wells of the 2019-nCoV marker protein-coated plate, add 3 duplicate wells for each sample, and set up 3 groups of negative controls and 3 groups of positive controls, seal the sealing film, and incubate in a 37°C incubator for 30 minutes;

[0074] S2. Wash the plate for the first time: Discard the liquid in the wells of the 2019-nCoV marker protein-coated plate incubated with the primary antibody, dilute the washing solution 10 times, and wash the plate 3 times;

[0075] S3. Enzyme label incubation: add 0.1mL anti-human IgA antibody enzy...

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Abstract

The invention relates to the technical field of biological medicines, and particularly discloses a 2019-nCoV IgG, IgM and IgA antibody combined detection kit and a detection method thereof. The 2019-nCoV IgG, IgM and IgA antibody joint detection kit comprises a 2019-nCoV marker protein coating plate, a sample diluent, a washing solution, an anti-human IgA antibody enzyme-labeled working solution,an anti-human IgG antibody enzyme-labeled working solution, an anti-human IgM antibody enzyme-labeled working solution, a chromogenic substrate, a stop solution and a positive control. The kit provided by the invention can simultaneously detect IgG, IgM and IgA antibodies of 2019-nCoV, solves the problems of poor accuracy and high false negative result existence rate of a single antibody detectionresult, greatly improves the detection efficiency, and has extremely high application value.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a combined detection kit for IgG, IgM and IgA antibodies of 2019-nCoV and a detection method thereof. Background technique [0002] Enzyme-Linked ImmunoSorbent Assay (Enzyme-Linked ImmunoSorbent Assay, ELISA) has now become a commonly used method in the field of analytical chemistry, and is an immunoassay technique developed on the basis of Immunoenzymatic Techniques. [0003] Coronaviruses are a family of viruses known to cause colds as well as diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The new coronavirus (2019-nCoV) is a new strain of coronavirus that has never been found in humans before. It belongs to the genus β-coronavirus, and its genetic characteristics are significantly different from the aforementioned MERS and SARS. Common signs after human infection with coronavirus include respiratory symptoms, fever, ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569G01N33/577G01N33/58G01N33/545G01N33/543
CPCG01N33/6854G01N33/56983G01N33/577G01N33/581G01N33/545G01N33/54393G01N2333/165G01N2469/20
Inventor 王晓倩周帅张大准赵国萍王晓东张付献
Owner 深圳爱信生物技术有限公司
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