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Method for detecting taurine in formula food for special medical purposes

A technology of formula food and detection method, which is applied in measuring devices, scientific instruments, instruments, etc., can solve the problems of single component, few impurities, and unsuitable taurine detection.

Inactive Publication Date: 2020-12-22
特康药业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, compared with the method provided by the national standard, the sensitivity and selectivity of the detection can be improved, but the detection limit still needs to be improved, and the matrix and ingredients of the functional beverage food are single, and there are few impurities. For the detection of taurine in special food Detection cannot adapt

Method used

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Experimental program
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Embodiment Construction

[0037] A detection method for taurine in formula food for special medical purposes:

[0038] principle:

[0039] The sample was dissolved in water, and the protein was precipitated with potassium ferrocyanide and zinc acetate. The supernatant was derivatized with dansyl chloride, and the derivative was subjected to C 18 Reverse-phase column separation. Detect with an ultraviolet detector (254nm) or a fluorescence detector (excitation wavelength: 330nm; emission wavelength: 530nm), and quantify with an external standard method.

[0040] Reagents and materials:

[0041] Unless otherwise specified, the reagents used in this method are of analytical grade, and the water is the first-grade water specified in GB / T6682. Acetonitrile: chromatographically pure. Glacial acetic acid. hydrochloric acid. Anhydrous Sodium Carbonate. Sodium acetate. Methylamine Hydrochloride (Methylamine Hydrochloride). Dansyl chloride (5-dimethylaminonaphthalene 1-sulfonyl chloride): chromatogra...

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PUM

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Abstract

The invention discloses a method for detecting taurine in formula food for special medical purposes, and relates to the technical field of food detection, and the method comprises the following steps:firstly, preparing a standard solution; preparing a sample; and finally analyzing a result. According to the method, a sample is dissolved with water, protein is precipitated by potassium ferrocyanide and zinc acetate, supernatant is taken to subjected to a derivative reaction by using dansyl chloride, a derivative is separated by using a C18 reversed-phase chromatographic column, and finally thetaurine content in the formula food for special medical purposes is detected by using a detector.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting taurine in formula food for special medical purposes. Background technique [0002] Special foods refer to foods that are specially processed according to special formulas to meet the physiological needs of certain special populations or the nutritional needs of patients with certain diseases. [0003] Taurine is a special amino acid, which is the most abundant in marine shellfish and is an essential nutrient element for the human body. It has anti-atherosclerosis, prevention of hyperlipidemia, cerebral thrombosis and other effects. The content of taurine accounts for 50% of amino acids in the heart. It has obvious protective effect on ischemic cardiomyocytes and inhibits the occurrence of arrhythmia. Taurine provides a new method for the treatment of viral myocarditis. [0004] In the prior art, compared with the method provided by the national st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/8679G01N2030/067
Inventor 张灵燕王伟伟山秀英丁元康赵慧戚海波李新艳
Owner 特康药业集团有限公司
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