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Method for preparing white-rot fungus solid culture medium using chrome leather shavings and application

A solid medium, white rot fungus technology, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of reducing the content of hydrolyzed protein chromium, and achieve low chromium content, small fluctuation, and system reaction. smooth effect

Active Publication Date: 2020-12-22
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the current dechroming method is difficult to completely remove chromium, it is an effective means to reduce the chromium content in hydrolyzed protein and control it in an appropriate range through certain methods, and then apply to the fermentation culture of specific microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of method utilizing chrome leather shavings to prepare white rot fungus solid culture medium, comprises the following steps:

[0038] (1) Hydrolytic dechromation: In a constant temperature water bath at 60°C, add 25g of chromium leather scraps (containing Cr 2 o 3: 3.2%), then add 3g of calcium oxide, 300g of deionized water, stir and react for 0.5h, add 12.5g of chrome leather shavings, continue to react for 0.5h, then add 12.5g of chrome leather shavings and continue to react for 1h to obtain a viscous The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction while it was hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, collect the l...

Embodiment 2

[0052] A kind of method utilizing chrome leather shavings to prepare white rot fungus solid culture medium, comprises the following steps:

[0053] (1) Hydrolytic dechromation: In a constant temperature water bath at 60°C, add 30g of chromium leather shavings (containing Cr 2 o 3 : 2.5%), then add 4.2g of calcium oxide, 390g of deionized water, stir and react for 0.5h, then add 15g of discarded chrome leather scraps, continue to react for 0.5h, then add 15g of chrome leather scraps and continue to react for 1h to obtain viscous The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction while it was hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, col...

Embodiment 3

[0067] A kind of method utilizing chrome leather shavings to prepare white rot fungus solid culture medium, comprises the following steps:

[0068] (1) Hydrolytic dechromation: In a constant temperature water bath at 80°C, add 20g of chromium leather scraps (containing Cr 2 o 3 : 5%), then add 3.6g of calcium oxide, 400g of deionized water, stir and react for 0.5h, then add 10g of chrome leather shavings, continue to react for 0.5h, then add 10g of chrome leather shavings and continue to react for 1h to obtain viscous The hydrolyzed protein homogeneous mixture was centrifugally filtered while it was hot to remove the insoluble solid matter, and the filtrate was collected. The hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, and the white precipitated calcium carbonate was removed by suction filtration, and the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, co...

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Abstract

The invention belongs to the field of leather solid wastes, and particularly relates to a method for preparing a white-rot fungus solid culture medium using chrome leather shavings and application. The method comprises the following steps of: firstly, preparing dechromed hydrolyzed collagen by using tanning waste chrome leather shavings as a raw material and adopting a batch feeding process basedon an alkaline enzyme hydrolysis system; secondly, compounding glucose, a small amount of peptone, a trace element solution, agar, vitamin B1 and purified water by taking the extracted dechromed hydrolyzed collagen as a nutrient source to prepare a solid fermentation culture medium; and finally, fermenting and culturing white-rot fungi by utilizing a solid culture medium. The prepared solid culture medium can achieve normal and efficient growth of the white-rot fungi, not only achieves the purpose of changing the waste chrome leather shavings into valuables, but also can greatly replace traditional peptone resources, effectively reduces the culture cost of the white-rot fungi, and is beneficial to high-valued utilization of chrome-protein-containing solid wastes.

Description

technical field [0001] The invention belongs to the field of solid leather wastes, and in particular relates to a method and application for preparing a solid medium for white-rot fungi by using chrome leather shavings. Background technique [0002] Since the 1990s, the leather industry has developed rapidly, and China has gradually become the largest leather-making country in the world, but the accompanying pollution problems have also become increasingly serious. Especially in the leather production process, 70% of the wastes are chromium-containing wastes, which mainly include chrome leather shavings, chrome-containing leather pieces, grinding leather ash, etc. Nitrogen sources, on the other hand, are the nutrients that constitute the source of nitrogen in microbial cells and metabolites. Tannery waste is rich in collagen and its hydrolysis products (polypeptides, amino acids, etc.) in different degrees, which can provide the necessary organic nitrogen source for the gro...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P21/06C12R1/645
CPCC12N1/14C12P21/06
Inventor 刘洁张斐斐徐洲李彦春李雪松周玉
Owner QILU UNIV OF TECH
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