Method for preparing white-rot fungus solid culture medium using chrome leather shavings and application
A solid medium, white rot fungus technology, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of reducing the content of hydrolyzed protein chromium, and achieve low chromium content, small fluctuation, and system reaction. smooth effect
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Embodiment 1
[0037] A kind of method utilizing chrome leather shavings to prepare white rot fungus solid culture medium, comprises the following steps:
[0038] (1) Hydrolytic dechromation: In a constant temperature water bath at 60°C, add 25g of chromium leather scraps (containing Cr 2 o 3: 3.2%), then add 3g of calcium oxide, 300g of deionized water, stir and react for 0.5h, add 12.5g of chrome leather shavings, continue to react for 0.5h, then add 12.5g of chrome leather shavings and continue to react for 1h to obtain a viscous The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction while it was hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, collect the l...
Embodiment 2
[0052] A kind of method utilizing chrome leather shavings to prepare white rot fungus solid culture medium, comprises the following steps:
[0053] (1) Hydrolytic dechromation: In a constant temperature water bath at 60°C, add 30g of chromium leather shavings (containing Cr 2 o 3 : 2.5%), then add 4.2g of calcium oxide, 390g of deionized water, stir and react for 0.5h, then add 15g of discarded chrome leather scraps, continue to react for 0.5h, then add 15g of chrome leather scraps and continue to react for 1h to obtain viscous The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction while it was hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, col...
Embodiment 3
[0067] A kind of method utilizing chrome leather shavings to prepare white rot fungus solid culture medium, comprises the following steps:
[0068] (1) Hydrolytic dechromation: In a constant temperature water bath at 80°C, add 20g of chromium leather scraps (containing Cr 2 o 3 : 5%), then add 3.6g of calcium oxide, 400g of deionized water, stir and react for 0.5h, then add 10g of chrome leather shavings, continue to react for 0.5h, then add 10g of chrome leather shavings and continue to react for 1h to obtain viscous The hydrolyzed protein homogeneous mixture was centrifugally filtered while it was hot to remove the insoluble solid matter, and the filtrate was collected. The hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, and the white precipitated calcium carbonate was removed by suction filtration, and the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, co...
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