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Photoelectrochemical aptamer sensor based on sensing separation strategy and preparation method and application of photoelectrochemical aptamer sensor

An aptamer sensor and photoelectrochemical technology, applied in the field of biosensors, can solve problems such as poor sensitivity and weak photoelectric response, achieve high photoelectric signal, simple preparation method, and improve the effect of photocurrent response

Active Publication Date: 2020-12-18
SHANDONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the P-type optoelectronic matrix uses holes as the main carriers, the electrons provided by the electron donor cannot be used, and the charge recombination rate of the P-type optoelectronic active material is higher than that of the N-type material, resulting in weak photoelectric response and low sensitivity. poor

Method used

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  • Photoelectrochemical aptamer sensor based on sensing separation strategy and preparation method and application of photoelectrochemical aptamer sensor
  • Photoelectrochemical aptamer sensor based on sensing separation strategy and preparation method and application of photoelectrochemical aptamer sensor
  • Photoelectrochemical aptamer sensor based on sensing separation strategy and preparation method and application of photoelectrochemical aptamer sensor

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Experimental program
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Effect test

Embodiment 1

[0066] Due to PEDOT / Bi 2 S 3 The photocurrent output of the / ZnO photoanode has an important influence on the detection sensitivity of the final prepared photoelectrochemical aptasensor, so the following is for PEDOT / Bi 2 S 3 / ZnO photoanode preparation process parameters were optimized:

[0067] 1) Due to Bi(NO 3 ) 3 Concentration of ethylene glycol solution versus Bi 2 S 3 The morphology and thickness of the nanosheets have an influence, so the Bi(NO 3 ) 3 The concentration of the ethylene glycol solution is optimized as follows:

[0068] Prepare ethylene glycol solution (25mL) with a concentration of 4.5mM thiourea and different concentrations of Bi(NO 3 ) 3 Ethylene glycol solution (25mL), wherein, the Bi(NO 3 ) 3 The concentration of the ethylene glycol solution is 1mM, 2mM, 3mM, 4mM and 5mM; then the above two solutions are added to 150ml of ethanol and stirred until the mixed solution is clear; then the prepared solution is added to 100ml of Teflon In the lin...

Embodiment 2

[0074] Since the electron transport ability and probe modification amount of the sensing cathode have a significant impact on the detection sensitivity and quantitative detection range of the final prepared photoelectrochemical aptasensor, the following process parameters for the preparation of the sensing cathode were optimized:

[0075] 1) Because the modification amount of RGO on the sensing cathode has a significant impact on the electron transport ability of the cathode, the preparation process parameters of the deposited RGO were optimized, as follows:

[0076] Using cyclic voltammetry (CV), the concentration of graphene oxide solution used for electrodeposition was 0.8mg / mL, the voltage was set between -1.3V and 0.6V, the scanning speed was set at 0.1V / s, and the cycle was different. After performing 10, 20, 30, 40, and 50 cycles respectively, RGO / ITO electrodes with different modification amounts were obtained.

[0077] It can be obtained by carrying out the photocurre...

experiment example 1

[0086] (1) Preparation of ZnO nanorod modified electrode, the specific preparation steps are as follows:

[0087] Conduct electrodeposition in an aqueous solution containing 6.3mM zinc nitrate and 6.3mM hexamethylenetetramine. During electrodeposition, keep the solution temperature at 80°C and the deposition potential at 1.0V; then remove the substrate from the solution and use deionized Rinse with water and blow dry with nitrogen.

[0088] Among them, the scanning electron microscope as attached Figure 5 As shown, a large number of uniform ZnO nanorods are arranged in a columnar shape with an average diameter of 80-90 nm.

[0089] (2) Bi 2 S 3 / ZnO modified electrode preparation, the specific preparation steps are as follows:

[0090] Prepare a solution of 4.5mM thiourea in ethylene glycol (25mL) and a concentration of 3.0mM Bi(NO 3 ) 3 ethylene glycol solution (25mL); then the above two solutions were added to 150 milliliters of ethanol and stirred until the mixed sol...

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Abstract

The invention belongs to the technical field of biosensors, discloses a photoelectrochemical aptamer sensor based on a sensing separation strategy and a preparation method and application of the photoelectrochemical aptamer sensor, and particularly discloses a high-performance photoelectrochemical aptamer sensor constructed based on a three-dimensional cross-linked heterojunction structure and thesensing separation strategy. The thrombin aptamer is fixed at the sensing cathode to specifically capture thrombin, so that the impedance of a sensing interface is changed, and the change of a photocurrent detection signal is caused. Based on the excellent photoelectric property of the photo-anode and the specific recognition of the sensing cathode, the sensor has high sensitivity and good selectivity on thrombin detection, an efficient preparation strategy is provided for improving the photoelectric property of the photo-anode, the detection sensitivity of the sensor on related disease markers can be effectively improved, and the method is suitable for market popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a photoelectrochemical aptamer sensor based on a sensing separation strategy. More specifically, it relates to a high-performance photoelectrochemical aptasensor based on a three-dimensional cross-linked heterojunction structure and a sensing separation strategy and its preparation method. Background technique [0002] With the rapid development of science and technology and the improvement of living standards, people pay more and more attention to their health. The development of new medical equipment that can monitor target biological or biochemical processes in actual samples has become one of the biggest challenges for social development. Among them, biosensors have proven to be one of the most sensitive analytical tools in studying the natural control mechanisms of different metabolic pathways and changes in physiological states. Biosensors are analysis and detection devic...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N33/543
CPCG01N27/305G01N27/308G01N27/3275G01N33/5438
Inventor 马林政王群孙纪夫张德权楚天舒刘青云
Owner SHANDONG UNIV OF SCI & TECH
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