Cell flow tank, cell detection device and cell detection method

A detection device and detection method technology, applied in the field of cell analysis, can solve the problems of unfavorable target cell analysis results, low efficiency, hinder mass spectrometry detection, etc., and achieve the effects of high ion transmission efficiency, high detection efficiency, and improved reception efficiency.

Pending Publication Date: 2020-12-11
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in mass spectrometry flow cytometry, after the cell sample is separated into individual cells by the flow chamber and the liquid flow system, all of them need to be subjected to mass spectrometry analysis in turn, which is not conducive to quickly obtaining the analysis results of the target cells, and the efficiency is low
In the flow mass spectrometry based on the laser ablation method, there is a phenomenon of excessive desorption of samples, and too many ions are generated per unit time, which causes saturation effects and hinders mass spectrometry detection

Method used

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  • Cell flow tank, cell detection device and cell detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A cell detection device, the cell detection device comprising:

[0037] a mass spectrometer having an inlet;

[0038] A sampling unit, the sampling unit adopts a cell flow cell, and the cell flow cell includes: a body, the body has an inlet and an outlet, and the inside has a plurality of flow channels suitable for cells to pass through and distributed in arrays suitable for containing cells The groove, the flow channel is used to communicate with adjacent grooves; the inlet and outlet are respectively connected to the groove, the depth of the groove is greater than the depth of the flow channel; a plurality of openings, a plurality of openings are The array is distributed on the upper end of the body and is located on the upper side of the groove; the groove communicates with the outside through the opening;

[0039] A detection unit, the detection unit is used to obtain the abnormal groove position of the cell on the sampling unit;

[0040] a plurality of electrodes...

Embodiment 2

[0055]An application example of the cell detection device and method according to Embodiment 1 of the present invention in the detection of red blood cells.

[0056] In this application example, the sampling unit (cell flow cell) includes the first layer, the second layer, the third layer and the fourth layer stacked from top to bottom, and the adjacent layers are connected by glue; the first layer has a Openings distributed in an array, the diameter of the cylindrical opening is smaller than the diameter of the groove; the side of the second layer has an inlet and an outlet, and the upper end has grooves distributed in an array, and the inner wall of the groove is a non-smooth surface, suitable for accommodating one or more a cell, the groove has a diameter R ∈ [10 μm, 100 μm], such as 10 μm, 30 μm, 50 μm, 80 μm, 100 μm, a depth H ∈ [5 μm, 50 μm], such as 5 μm, 12 μm, 24 μm, 35 μm, 50 μm, adjacent The grooves communicate with each other through flow channels, the depth of the...

Embodiment 3

[0069] According to the application example of the cell detection device and method of embodiment 1 of the present invention, the difference from embodiment 2 is:

[0070] 1. The opening of the first layer is frustum-shaped, and the diameter of the opening becomes larger from top to bottom, wherein the smallest diameter is smaller than the diameter of the groove, and the largest diameter is not larger than the diameter of the groove.

[0071] 2. The angle between the transmission pipe and the upper end surface of the first layer is 90 degrees.

[0072] 3. The light reflection layer is no longer provided separately, but the light reflection film formed on the bottom wall of the groove is used.

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Abstract

The invention provides a cell flow tank, a cell detection device and a cell detection method. The cell flow tank comprises a body; the body is provided with an inlet and an outlet; the interior of thebody is provided with a plurality of flow channels suitable for cells to pass through and grooves which are distributed in an array mode and suitable for containing the cells, wherein the flow channels are used for communicating the adjacent grooves; the inlet and the outlet are communicated with the grooves, and the depth of the grooves is greater than that of the flow channels; a plurality of openings are distributed at the upper end of the body in an array manner and are positioned on the upper sides of the grooves; and the grooves are communicated with the outside through the openings. The cell flow tank has the advantages of accurate detection, automation and the like.

Description

technical field [0001] The invention relates to cell analysis, in particular to a cell flow cell, a cell detection device and a method. Background technique [0002] Single-cell technology has developed rapidly in recent years. With the continuous development of technologies such as single-cell sequencing and single-cell transcriptome, humans have gained a deep understanding of the heterogeneity of cell populations from the level of DNA and RNA. At the protein level, flow cytometry has always been the most commonly used single-cell analysis method, but due to problems such as cross-color, flow cytometry usually can only detect 6-10 kinds of proteins at the same time. [0003] Mass cytometry (Mass Cytometry) is a flow cytometry technique that uses the principle of mass spectrometry to perform multi-parameter detection of single cells. It inherits the high-speed analysis characteristics of traditional flow cytometry and has the high-resolution capability of mass spectrometry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N15/10
CPCG01N15/1031G01N15/1404G01N15/1429G01N2015/0065G01N2015/1006
Inventor 闻路红余晓梅胡舜迪毕磊陈安琪施露露
Owner NINGBO UNIV
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