A method and kit for rapidly extracting Mycobacterium tuberculosis nucleic acid from sputum

Abstract

The invention relates to the field of biomedical clinical diagnostic reagents, in particular to a kit for rapidly extracting Mycobacterium tuberculosis nucleic acid from sputum, comprising a lysate, a magnetic bead suspension, a cleaning solution and an eluent; the lysate includes 3-5mol / L potassium chloride, 3-5mol / L guanidine hydrochloride, 1% potassium ethyl xanthate and 0.2mol / L potassium phosphate buffer; the magnetic bead suspension is a nucleic acid composed of polyethyleneimine-modified magnetic beads Adsorption carrier; the cleaning solution is a hydrochloric acid solution; the eluent is a sodium hydroxide solution; a method for rapidly extracting Mycobacterium tuberculosis nucleic acid in sputum is further proposed, so as to finally realize high-quality and fast extraction of Mycobacterium tuberculosis nucleic acid in sputum Extraction and detection, reduce the cost of extraction, simplify the operation process, avoid the use of volatile organic solvents and high-concentration salt solutions, and reduce the hazards of kit reagents to the health of operators. At the same time, the kit is combined with existing automatic detection technology to achieve Potential for high-throughput, large-scale nucleic acid extraction and detection.

Description

technical field [0001] The invention relates to the field of biomedical clinical diagnostic reagents, in particular to a method and a kit for rapidly extracting Mycobacterium tuberculosis nucleic acid from sputum. Background technique [0002] Mycobacterium tuberculosis (Mycobacterium tuberculosis, referred to as Mycobacterium tuberculosis) is the pathogen that causes tuberculosis. This bacterium can infect the whole body tissues and organs of the human body, mainly the lungs, and poses a major threat to human life and health. With the emergence of anti-tuberculosis drugs and BCG vaccine and the improvement of medical level, the infection of Mycobacterium tuberculosis once dropped sharply. However, due to the abuse of antibiotics, the emergence of drug-resistant strains and the prevalence of AIDS, the prevalence of tuberculosis worldwide tends to worsen. According to World Health Organization estimates, in 2018, there were about 10 million new cases of Mycobacterium tubercu...

AI Technical Summary

Problems solved by technology

"Nucleic acid extraction composition and its application, reagents and kits containing the nucleic acid extraction composition" (Patent No.: CN202010347016.5) uses traditional silica-based adsorption columns, magnetic beads or glass beads as nucleic acid carriers for purification, after cleaning Different concentrations of salt ions introduced into the solution and strengthening solution may contaminate the subsequent nucleic acid purification risk

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