Heparin sodium production equipment based on secondary enzymolysis method

Abstract

The invention discloses heparin sodium production equipment based on a secondary enzymolysis method. The heparin sodium production equipment comprises a cylinder body, supporting legs, a stirring device, a cover plate, a first rotating piece and a detection structure, the stirring device comprises a stirring cavity formed in the cylinder body, a first cavity formed in the inner wall of the cylinder body, a heating pipe arranged in the first cavity, a motor arranged on the cover plate, a telescopic stirring structure arranged below the cover plate, and a first auxiliary structure arranged on the side wall of the stirring cavity; the device also comprises a discharging structure arranged at the bottom of the stirring cavity; through the arrangement of the telescopic stirring structure, a stirring shaft can be contracted after stirring is completed, and the stirring shaft is opened in the form of a flip cover, so that the design height of a tank body is higher, an occupied area is reduced, accommodating volume of equipment is increased, and the interior of the equipment is more convenient to clean; Heating efficiency is effectively guaranteed in a side wall heating mode, internal temperature balance is guaranteed, and preparation efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to a production equipment for heparin sodium based on a secondary enzymatic hydrolysis method. Background technique [0002] In the process of preparing heparin sodium, add the prepared porcine intestinal mucosa raw material into the sodium chloride aqueous solution with a salinity of 4.0 to 4.5 degrees, mix evenly to obtain a mixed solution, and add a pH regulator to adjust the pH of the mixed solution. pH value, and the temperature of the mixture was raised to 33±2°C, then alkaline protease was added, the temperature was continuously raised to 38±2°C, and then the enzymolysis was kept at a constant temperature for 1-1.5 hours to complete the first enzymolysis. [0003] After the first enzymatic hydrolysis, add a pH regulator to adjust the pH value of the mixed solution, adjust the salinity of the mixed solution to 3.0-3.5 degrees, continue to heat up to 55±1℃, an...

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Problems solved by technology

[0004] In the process of enzymatic hydrolysis, a certain degree of heat preservation and stirring is required, so stirring equipment is required. After the preparation, the equipment needs to be cleaned to avoid the growth of internal bacteria. Conventional equipment on the market will be stirred during the cleaning process. During the process of taking out the shaft, due to the long length of the stirring shaft, it is necessary to lift the stirring shaft vertically upwards to remove the entire stirring shaft from the barrel. The stirring shaft can only be taken out by climbing a ladder. On the one hand, it is inconvenient to clean. On the other hand, climbing high is dangerous to workers. The mixing equipment on the market also has a stirring shaft in the form of a flip cover, but the flip cover requires an opening. The size of the mixer is matched with the length of the stirring shaft, so the stirring devices on the market are usually short in height, occupy a large area, but have a small capacity. Due to the long preparation time and low output, the heating method usually adopts Water bath heating method, but the heating tubes are all set at the bottom, and the heating process is from bottom to top, resulting in a large temperature difference between the upper and lower sides, which may easily lead to subsequent uneven temperature

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