Method for rhizome plant test-tube plantlet transplanting

Abstract

The invention discloses a method for rhizome plant test-tube plantlet transplanting. The method comprises the following steps that S1, seedling hardening culture is carried out on tissue culture seedlings of rhizome plants; S2, cleaning, disinfecting and airing are carried out on rhizome test-tube plantlets; S3, grading planting is carried out according to the growth condition of the rhizome test-tube plantlets; S4, cultivation management is carried out after transplanting; and S5, seedling formation and outplanting of the rhizomes test-tube plantlets are carried out. According to the method,sterilization rooting powder is introduced in the transplanting process, and used when polygonatum cyrtonema tissue culture industrialized seedling rhizome test-tube plantlets are transplanted, the high survival rate and the seedling rate are obtained, rooting of rhizomes and germination and growth of buds are accelerated, the efficient sterilization effect is achieved, rotting of the rhizomes andbiting of soil insects are effectively prevented, rooting and seedling formation of the rhizomes are guaranteed, the transplanting survival rate of the rhizome test-tube plantlets is increased, and the method can be also suitable for other meristematic propagation rhizome plants.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and more specifically relates to a method for transplanting rhizome plantlets in test tubes. Background technique [0002] Rhizome plants (such as: Polygonatumcyrtonema Hua, Solanumtuberosum L., Zingiber officinale, Dioscorea Zingiberensis C.H.Wright, Dioscorea oppositifolia L.) generally flower but do not set seeds, or have few seeds, and it is difficult to germinate . Thereby it is difficult to adopt seeds to carry out large-scale seedling production. At present, except for potatoes, most other varieties cannot adopt plant tissue culture technology for mass propagation of seedlings. Rhizome meristematic propagation is a relatively common propagation method at present, but this propagation method needs to consume a large amount of original rhizome material, and the seedlings cultivated by this method are easy to degenerate, and due to virus infection, it seriously affects the subsequ...

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Problems solved by technology

But no one has successfully carried out it at present, mainly because the morphological structure of Polygonatum multiflora plants is special, the rhizome flesh grows in the soil, the explants are difficult to sterilize and become sterile propagation materials, and the rhizomes contain Rich in polysaccharides and other metabolic secondary substances, it is easy to brown, and Polygonatum polyflora has the biological characteristics of dormancy. It is very difficult to adopt the bud proliferation method like most plant tissue cultures to continuously expand the number of buds.

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