Method for simultaneously extracting heparin sodium and intestinal membrane protein peptide based on enzymolysis method

Abstract

The invention relates to a method for simultaneously extracting heparin sodium and intestinal membrane protein peptide based on an enzymolysis method. The method comprises the following steps of: preparing an intestinal mucosa mixed solution; preparing enzymatic hydrolysate by adopting an enzymolysis method; filtering to obtain clarified filtrate and an enzymolysis concentrated solution; performing heparin adsorption on the filtrate through an ion exchange resin column, and obtaining effluent at the same time; taking down the adsorbed and saturated ion exchange resin column, firstly cleaning the resin in the ion exchange resin column by using cleaning saline water, and then performing heparin elution on the resin by using desorption saline water to obtain heparin sodium eluent; filtering,desalting and concentrating the heparin sodium eluent to obtain a heparin sodium concentrated solution; drying the heparin sodium concentrated solution to obtain crude heparin sodium; cooling the effluent, and then carrying out desalination and concentration to obtain a first concentrated solution; carrying out vacuum concentration on the first concentrated solution to obtain a protein peptide concentrated solution; and drying the protein peptide concentrated solution to obtain the protein peptide. The method is high in heparin sodium extraction rate, short in production period, capable of extracting protein peptide at the same time, low in cost and low in pollution.

Description

technical field [0001] The invention relates to a method for extracting heparin sodium, in particular to a method for simultaneously extracting heparin sodium and intestinal membrane protein peptides based on an enzymatic hydrolysis method. Background technique [0002] At present, there are two techniques for extracting heparin in China: salting-out method and enzymatic hydrolysis method. The salting-out method is to add salt and alkali to the intestinal mucosa to adjust the pH, raise the temperature to about 50°C and keep warm to dissolve the heparin and transfer it to water, then raise the temperature to 95°C to solidify the intestinal membrane protein, and then separate to obtain the by-product intestinal membrane slag (or called Intestinal membrane protein), and the separated supernatant is subjected to resin adsorption, analysis, alcohol precipitation, drying and other steps to obtain heparin. The enzymatic hydrolysis method is to add salt to the intestinal mucosa, ad...

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Problems solved by technology

[0007] Large amount of wastewater: Traditional industrial production methods have a large amount of wastewater discharge and high salt content, especially the organic matter content in the discharge water of the enzymatic hydrolysis process is very high, the wastewater treatment is difficult and the operating cost is high;

[0008] A large amount of dangerous organic solvents are used: the traditional production method adopts ethanol precipitation and standing overnight to collect heparin, which has a long production cycle, high requirements for workshop level, strict operation and management requirements for ethanol recovery and storage, and high cost

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