Extraction and purification method of lithocarpus litseifolius polysaccharide

An extraction method and technology of wood ginger, which is applied in the field of extraction and purification of polysaccharides from leaves of wood ginger, can solve problems such as extraction methods of polysaccharides from leaves of wood ginger that have not been reported, achieve stable and reliable extraction process, high-efficiency extraction of polysaccharides, and reduce surface tension and viscosity Effect

Pending Publication Date: 2020-11-27
CHENGDU AGRI SCI & TECH CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report on the extraction method of coylea polysaccharide

Method used

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  • Extraction and purification method of lithocarpus litseifolius polysaccharide
  • Extraction and purification method of lithocarpus litseifolius polysaccharide
  • Extraction and purification method of lithocarpus litseifolius polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Extraction of Polysaccharide

[0030] (1) Dry the finished Mujiangyeke tea leaves in a constant temperature hot air oven at 45°C to constant weight, then grind them with a Super Mill-1500 powder machine, then pass through a 60-mesh sieve, put the Mujiangyeke tea powder into a sealed bag, and place Store at -20°C for later use.

[0031] (2) Accurately weigh 2g of Mujiang Yeke tea powder, add it to a 50mL centrifuge tube, and vortex to mix, add 20mL of 80% ethanol (1:10, w / v), use PL-S80T ultrasonic cleaning machine at 640W power Ultrasound for 30min. After the sample was balanced, it was centrifuged at 4000g for 10min by an X3R high-speed refrigerated centrifuge, and the supernatant was discarded, then 20mL of 80% ethanol was added, and the PL-S80T ultrasonic cleaner was used to sonicate for 30min at 80% power. After the sample was balanced, it was centrifuged at 4000g for 10min by an X3R high-speed refrigerated centrifuge, and the supernatant was discarded t...

Embodiment 2

[0039] Example 2 Extraction of Polysaccharides from Leaf Ginger Leaf Kecha

[0040] The difference with Example 1 is: the solid-liquid ratio when adopting high-pressure extraction in step (3) is 1:24, the extraction time is 41min, and the extraction pressure is 0.09Mpa; the rest are the same as Example 1.

[0041] The extraction rate of polysaccharides in this example is 4.65%, and the extraction yield of polysaccharides is 93.0 mg.

Embodiment 3

[0042] Example 3 The Extraction of Polysaccharides from Lemon Ginger Leaf Ke Tealeaves

[0043] The difference with Example 1 is: the solid-liquid ratio when adopting high-pressure extraction in step (3) is 1:20, the extraction time is 40min, and the extraction pressure is 0.02Mpa; the rest are the same as in Example 1.

[0044] After testing, the extraction rate of this embodiment is 3.29%, and the extraction yield of polysaccharide is 65.8 mg.

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Abstract

The invention discloses an extraction and purification method of lithocarpus litseifolius polysaccharide. The method comprises the following steps of: (1) pretreating lithocarpus litseifolius leaves to obtain lithocarpus litseifolius leaf powder; (2) removing fat-soluble compounds in the lithocarpus litseifolius leaf powder; (3) with water adopted as an extraction solvent, carrying out pressurizedextraction on the lithocarpus litseifolius leaves from which the fat-soluble compounds are removed; and (4) centrifuging an extract, and taking a supernatant to obtain the lithocarpus litseifolius leaf crude polysaccharide extract. According to the method, the polysaccharide is extracted from the lithocarpus litseifolius leaves by adopting the pressurized solvent-assisted extraction method, the optimal extraction process parameters for extracting the lithocarpus litseifolius leaf polysaccharide by adopting the pressurized solvent are optimized by combining a single-factor multi-level experiment with a Box-Behnken center combination design-response surface method, and therefore, the purpose of efficiently extracting the polysaccharide is achieved. The method is high in extraction efficiency, low in extraction cost, short in extraction time, low in energy consumption, simple in extraction process, stable, reliable and suitable for popularization and application.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from plants, in particular to a method for extracting and purifying polysaccharides from curly polysaccharides, and belongs to the field of polysaccharide extraction and purification. Background technique [0002] Lithocarpus litseifolius (Hance) Chun (scientific name: Lithocarpus litseifolius (Hance) Chun) is an arbor of the Fagaceae family and is distributed in various provinces and regions south of the southern slope of the Qinling Mountains in China. The young leaves have a sweet taste, and when chewed, they are viscose. Most mountain residents in the south of the Yangtze River use their leaves as tea substitutes, commonly known as sweet tea. Mujiangye Ketian tea has been eaten by Chinese people for thousands of years. It is a kind of plant that has both medicine and food, and has three functions of tea, sugar and medicine. Among them, Sichuan, Hunan and Yunnan are the most abundant r...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 甘人友吴定涛郭欢夏宇刘宏艳刘毅
Owner CHENGDU AGRI SCI & TECH CENT
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