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Biliary atresia diagnostic kit

A diagnostic kit and biliary atresia technology, applied in the field of medical diagnostics, can solve the problems of lack of early diagnostic indicators and low cure rate in children with BA, and achieve the effect of promoting and using

Inactive Publication Date: 2020-11-24
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, children with BA lack effective early diagnosis indicators and detection methods, and the discovery is in the middle and late stage, so the cure rate is very low, and the children also have different degrees of clinical symptoms after surgery.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] 1) Sorting of CD177+ cells in peripheral blood

[0044]The erythrocytes in peripheral blood of children in NC group and BA group were lysed with erythrocyte lysate to make single cell suspension for later use. Add FcR blocking to the cell suspension and incubate at room temperature for 10min, add anti-human PE conjugated CD177 antibody and incubate at 4°C for 30min, wash with flow staining buffer twice, add anti-PE Microbeads and incubate at 4°C for 15min, wash with flow staining buffer Twice, LD magnetic separation column was used to carry out the sorting of CD177+ cells on a magnetic cell sorter (MiltenyiBiotec, Germany), and the sorted CD177+ cells were tested for cell purity on a BD FACS Aria II (BD Biosciences, USA), And use cells with cell purity>95% for the next experiment.

[0045] 2) Electron microscope observation and analysis of mitochondria in CD177+ cells

[0046] Take 1.5×10 respectively 6 A CD177+ cell was selected from the peripheral blood samples of ...

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Abstract

The invention relates to the field of medical diagnostics, in particular to a biliary atresia diagnostic kit, which comprises a CD177 + neutrophil sorting reagent and a reagent for observing mitochondrial morphology and / or judging whether mitochondria has dysfunction or not. According to the kit provided by the invention, a specimen required for detection is obtained during normal examination, sothat a patient does not need to be additionally subjected to traumatic detection, and the kit is beneficial to popularization and application in the field of biliary atresia detection.

Description

technical field [0001] The invention relates to the field of medical diagnostics, in particular to a diagnostic kit for biliary atresia. Background technique [0002] Biliary atresia (BA) is a progressive, idiopathic, fibro-occlusive extrahepatic biliary system disease that occurs only in the neonatal period, manifesting as biliary obstruction. The overall incidence of biliary atresia is low, 1 / 20,000-1 / 10,000 live births, but it is the most common cause of neonatal jaundice requiring surgery and the most common indication for liver transplantation in children. The prognosis of biliary atresia is poor, the mortality rate is high, and the etiology is still unknown. The basic pathological changes are progressive inflammation of extrahepatic and intrahepatic bile ducts and liver fibrosis. Most of the children were found 1 to 3 months after birth, and most of them died of liver failure within 1.5 years because they could not perform liver transplantation. At present, due to t...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531G01N33/533G01N33/543
CPCG01N33/53G01N33/531G01N33/533G01N33/543
Inventor 张锐忠陈虹交苏亮付铭陈严夏慧敏
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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