Functionally linked polypeptide, exosome drug delivery system that can specifically bind collagen and its application

A technology for connecting peptides and exosomes, which is used in the repair of spinal cord injury. It can specifically bind to collagen exosome drug delivery system and its construction field. It can solve the problem of limited exosome microspheres and exosome microspheres. It can reduce the rapid loss of balls, promote the recovery of behavioral functions, and promote maturity and functionalization.

Active Publication Date: 2022-06-17
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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Problems solved by technology

However, if the drug-loaded exosomes and collagen scaffolds only rely on simple physical adsorption, although there is a certain local enrichment effect, there is still an uncontrollable loss of exosome microspheres in the damaged area, and eventually they can be recovered in the injured area. Locally acting exosome microspheres are limited

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  • Functionally linked polypeptide, exosome drug delivery system that can specifically bind collagen and its application
  • Functionally linked polypeptide, exosome drug delivery system that can specifically bind collagen and its application
  • Functionally linked polypeptide, exosome drug delivery system that can specifically bind collagen and its application

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Embodiment Construction

[0039] In view of the deficiencies in the prior art, the inventors of the present invention have, through long-term research and extensive practice, connected an exosome drug-carrying system with collagen-specific binding ability to a collagen scaffold. The technical solution, its implementation process and principle will be further explained as follows.

[0040] One aspect of the embodiments of the present invention provides a functionally linked polypeptide comprising a collagen-binding peptide sequence and an exosome-binding peptide sequence linked to the collagen-binding peptide sequence.

[0041]In some preferred embodiments, the functionally linked polypeptide has an exosome-binding peptide sequence (BP9) at one end that specifically binds to the surface of drug-loaded exosomes, and a collagen-binding peptide sequence (CBD) that specifically binds to the surface of collagen at the other end. Double binding function.

[0042] Further, the functional linking polypeptide (...

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Abstract

The invention discloses a functional linking polypeptide, an exosome drug-carrying system capable of specifically binding to collagen and applications thereof. The functional linking polypeptide comprises a collagen-binding peptide sequence and an exosome-binding peptide sequence connected to the collagen-binding sequence, and has the amino acid sequence shown in SEQ ID NO:1. The exosome drug-carrying system that can specifically bind to collagen comprises: drug-loaded exosomes, and a functional linker polypeptide that specifically binds to the drug-loaded exosomes. The exosome drug-carrying system that can specifically bind to collagen constructed by the present invention can effectively connect drug-loaded exosomes to collagen materials, reduce the rapid loss of drugs after transplantation, and have the ability to maintain the concentration of drugs in the injured part and slow down The release ability can prolong the drug half-life, promote more neural stem cells to differentiate into neurons, and mature into functional neurons. Promote the production of more nerve fibers, reduce scar formation in the injured area, and promote better motor function recovery in spinal cord injuries.

Description

technical field [0001] The invention belongs to the technical fields of cell biology, molecular biology and drug research and development, in particular to an exosome drug microsphere with collagen-specific binding ability, in particular to an exosome drug-carrying system that can specifically bind to collagen and Its construction method, and its application in spinal cord injury repair. Background technique [0002] Spinal cord injury is a serious central nervous system injury disease. After injury, a large number of neurons and axons are lost, and a series of pathological changes can be caused, including vascular system decomposition, edema, persistent inflammatory response, and scarring. Due to the production of a large number of inhibitory molecules and the deposition of glial scars, a microenvironment unfavorable for regeneration is formed at the injury site, resulting in the differentiation of neural stem cells into glial cells and blocking axon regeneration. In recen...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61K47/42A61K47/46A61K31/337A61P25/28
CPCC07K14/47A61K47/42A61K47/46A61K31/337A61P25/28C07K2319/33
Inventor 戴建武陈艳艳张璐璐
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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