Molecular marker combination for identifying Russian dandelion and application of molecular marker combination

Abstract

The invention relates to the technical field of molecular markers, and in particular relates to a molecular marker combination for identifying Russian dandelion and application of the molecular markercombination. In the invention, a method for identifying and distinguishing Russian dandelion is designed according to three SSR markers ONT21, ONT876 and ONT15498 in dandelion plant gene, specifically, corresponding specific primers can be designed for the three SSR markers, then samples to be identified are subjected to PCR reaction, and the species type of the material to be identified can be determined through the amplified bands. The specific primers designed by the invention have high specificity and sensitivity, and the accuracy of amplification results can be ensured. The identification method of Russian dandelion based on three SSR markers provided by the invention can accurately identify and distinguish Russian dandelion from various dandelions of the same genus and different species, effectively prevent interspecific hybridization, and has great significance in the breeding and genetic protection of Russian dandelion.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a combination of molecular markers for identifying rubber grass and its application. Background technique [0002] Rubber grass, also known as Russian dandelion (Taraxacum kok-saghyz Rodin, TKS), is a perennial herb belonging to the family Asteraceae. Research has found that rubber grass contains 4-5% high-quality natural rubber, which mainly exists in the roots of rubber grass, and can be used to make various rubber products. It is a rubber-producing plant with high economic value in the prior art. [0003] Currently, rubber grass is mostly mixed with other plants of the genus Taraxacum, such as medicinal dandelion (Taraxacum officinale F.H.Wigg., TO), common dandelion (Taraxacum mongolicum Hand.-Mazz, TM) and short-horned dandelion ( Taraxacum brevicorniculatum Korol., TB). The phenotypes of these Dandelion plants are relatively close, and it is difficult to disting...

AI Technical Summary

Problems solved by technology

The phenotypes of these Dandelion plants are relatively close, and it is difficult to distinguish and identify them based on phenotype alone, which brings great difficulties to the collection and identification of rubber grass in the field

At the same time, rubber grass and common dandelion can interbreed, which brings a lot of trouble to the planting preservation and genetic protection of rubber grass

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