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Penicillium simplicissimum NL-Z1 bacteria for promoting growth of root nodules and improving abundance of probiotic microbial populations

A probiotic microorganism and Penicillium spp

Active Publication Date: 2020-11-20
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plant measures have become an important method for ecological restoration in areas with poor natural conditions such as bare rock slopes. However, due to site conditions, plants cannot take root in this harsh ecological environment, and it is difficult to achieve long-term protection.

Method used

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  • Penicillium simplicissimum NL-Z1 bacteria for promoting growth of root nodules and improving abundance of probiotic microbial populations
  • Penicillium simplicissimum NL-Z1 bacteria for promoting growth of root nodules and improving abundance of probiotic microbial populations
  • Penicillium simplicissimum NL-Z1 bacteria for promoting growth of root nodules and improving abundance of probiotic microbial populations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1 sample source

[0036] Strains were screened from rock samples from a bare rock slope located on Yueyang Avenue, Yueyang City, Hunan Province, China. Rock samples were collected from the upper, middle and lower parts of the slope, and brought back to the laboratory for processing, for compositional analysis of mineral samples and subsequent tests. According to the results of mineral analysis, the main components of the rock samples include the following: K 2 O 3.71%, Na 2 O 1.39%, CaO 0.21%, MgO 1.28%, P 2 o 5 0.11%, Fe 2 o 3 6.81%, Al 2 o 3 15.21%, MnO 0.04%.

[0037] 2 Separation and screening

[0038] 2.1 Medium

[0039] (1) Strain isolation medium: NaCl 0.3g, KCl 0.3g, (NH 4 ) SO 2 0.5 g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.3g, Ca 3 (PO 4 ) 25.0g, sucrose 10g, agar 15-20g, deionized water 1000mL, pH 7.0-7.5.

[0040] (2) PDA solid medium: potato 200g, glucose 20g, agar 18g, water 1000mL, pH7.0-7.2.

[0041] (3) Beef ext...

Embodiment 2

[0061] Investigate the effect of bacterial growth-promoting effect. Combined with the pot experiment, by planting horse thorn and observing its growth, the screened strains were tested more comprehensively and closer to practical applications, so as to explore the potential effects that can be exerted in actual environmental restoration and production applications.

[0062] Preparation of bacterial agents containing NL-Z1:

[0063] After activating the strain, inoculate it into a liquid medium and ferment for 3 days, and measure the OD with an ultraviolet spectrophotometer 600 , ensure the OD of the bacterial solution by diluting or continuing to ferment 600 The value is in the range of 0.8 to 1.2, and then sealed and stored in a refrigerator at 4°C for use.

[0064] When applying bacteria to potted plants, dilute the stored bacterial solution 100 times, put 60mL diluted bacterial solution into each pot, set up 3 parallels for each treatment, and use sterile medium as a blan...

Embodiment 3

[0084] Explore the effect of bacterial strains on microbial community composition.

[0085] The collected and sorted horsethorn rhizosphere soil samples were sent to Shanghai Majorbio Bio-pharm Technology Co., Ltd (Shanghai Majorbio Bio-pharm Technology Co., Ltd) for sequencing using the IlluminaMiseq platform.

[0086] Detection of microbial diversity in potting soil by high-throughput sequencing, and analysis of microbial community composition in potting soil. Depend on Figure 7 It can be seen that there is no difference in the microbial community composition at the phylum level between the control group and the potting soil treated with the NL-Z1 strain, mainly Ascomycota and Mortierellomycota. However, there are certain differences in the relative abundance of microorganisms between the groups. After NL-Z1 treatment, Ascomycota decreased from 85.32% to 1.32%, while Mortierellomycota increased from 12.81% to 94.95%, becoming the dominant phylum in the NL-Z1 treatment grou...

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Abstract

The invention discloses penicillium simplicissimum sp. NL-Z1 capable of promoting the growth of root nodules and improving the abundance of probiotic microbial populations, and belongs to the technical field of microorganisms. The penicillium simplicissimum NL-Z1 provided by the invention can be used for promoting the production of Indigofera pseudotinctoria, promoting the proliferation of root nodules of the plants and improving probiotic microorganisms and a nutritional environment. After the penicillium simplicissimum NL-Z1 provided by the invention is applied, nutrient elements such as potassium, calcium and magnesium required by plants in rock powder are effectively released; a spray-seeding matrix and the rock surface are promoted to be fused, nutrients are continuously supplied to plants, the total weight of root nodules is promoted to be increased by 152.63%, the hydrolytic nitrogen content is improved to be increased by 15.28%, and the symbiotic nitrogen fixation capacity is effectively exerted. Meanwhile, the relative abundance of Mortierella in soil is increased from 12.81% to 94.95%, and the increased quantity of the relative abundance of the Mortierella and the promotion effect on plants are superior to the effect of direct application of the Mortierella.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Penicillium simpleum NL-Z1 bacterium that promotes root nodule growth and increases the abundance of probiotic microbial populations. Background technique [0002] Plant measures have become an important method for ecological restoration in areas with poor natural conditions such as bare rock slopes. However, due to the limitations of site conditions, plants cannot take root in this harsh ecological environment, and it is difficult to achieve long-term protection. The application of microbial agents has received extensive attention in recent years. Microorganisms play an important role in mineral weathering, improving soil environment and regulating biological growth, and have a positive impact on ecological restoration. Leguminous shrubs have always been popular tree species in ecological restoration, so the growth promotion and growth promotion mechanism of legumes and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/36A01P21/00C09K17/14C09K101/00
CPCA01N63/36C09K17/14C09K2101/00C12N1/145C05F11/08C12R2001/80
Inventor 庄家尧刘超徐童心杨皓张文韬
Owner NANJING FORESTRY UNIV
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