Antrodia camphorata culture medium and preparation method thereof

Abstract

The invention belongs to the technical field of fungus bioengineering, and particularly relates to an antrodia camphorata culture medium and a preparation method thereof. The culture medium provided by the invention is prepared from the following raw materials including 30 g-60 g of a grain substrate, 0.3 g-0.6 g of gypsum powder, 20 ml-60 ml of a nutrient solution, 10 ml-30 ml of a shake flask strain and 0.2 ml-0.6 ml of an exogenous additive; the exogenous additive is soybean oil; and the shake flask strain is obtained by culturing carrots. The culture medium disclosed by the invention selects the grain substrate with lower price, particularly wheat grains, is suitable for growth of antrodia camphorata, is simple to prepare, and is suitable for large-scale culture and production of enterprises; the soybean oil is selected as the exogenous additive of the culture medium, the cost performance is high, the absorption and utilization rate is high, and the content of triterpenoids in theantrodia camphorata can be increased; and the carrots are selected as a shake flask nutrient solution to replace traditional potatoes, a constant temperature-cooling-heating three-stage culture manneris adopted, and growth and germination of antrodia camphorata mycelia are facilitated.

Description

technical field [0001] The invention belongs to the technical field of fungal bioengineering, and in particular relates to a culture medium of Antrodia camphorata and a preparation method thereof. Background technique [0002] Antrodia Cinnamomea belongs to the order Aphyllophyllum, Polyporaceae, and the genus Leptinosa. It is a unique and rare medicinal fungus discovered by scientists in Taiwan, China in 1990. It grows in mountainous areas at an altitude of 450-2000 meters, and only grows on the inner wall of the rotten heartwood of the trunk of the unique camphor tree more than a hundred years old in Taiwan. It is difficult to collect because the surface of the trunk is wet. At present, Taiwan calls it the most expensive wild fungus, Taiwan folks call it "the ruby ​​in the forest", and it is also called "Shenzhi" in Hong Kong and Macao. [0003] Antrodia Antrodia is rich in physiologically active ingredients, including polysaccharides, triterpenoids, superoxide dismutase,...

AI Technical Summary

Problems solved by technology

Basswood culture method can cultivate fruiting bodies of Antrodia camphorata that are similar to the active ingredients of wild Antrodia camphorata, but the cost is high and the cycle is slow, which cannot meet the market demand

The liquid culture method is an effective way to achieve industrial production of many medicinal fungi. The main advantages are short cycle and controllable quality. However, a large number of experiments by scientific research institutions have proved that the content of active ingredients in this method is low; the solid culture method has a moderate growth cycle. , but the preparation process of the space bag is complicated and the cultivation cost is high

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