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Multi-cell co-culture model based on fiber hydrogel and preparation method of model

A hydrogel and co-cultivation technology, applied in cell culture supports/coatings, biochemical equipment and methods, animal cells, etc., can solve the problem that tissue engineering scaffolds cannot achieve multi-cell co-cultivation, and achieve good mechanics High performance, simple preparation, and good biocompatibility

Pending Publication Date: 2020-11-06
GENERAL HOSPITAL OF PLA +1
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Problems solved by technology

[0003] The purpose of the present invention is to provide a multi-cell co-culture model based on fiber hydrogel and its preparation method, to solve the problem that the tissue engineering scaffolds prepared by traditional methods cannot realize the co-culture of multiple cells

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  • Multi-cell co-culture model based on fiber hydrogel and preparation method of model
  • Multi-cell co-culture model based on fiber hydrogel and preparation method of model

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with the accompanying drawings and embodiments. These more detailed descriptions are intended to help the understanding of the present invention and should not be used to limit the present invention. From the present disclosure it will be apparent to those skilled in the art that the present invention may be practiced without some or all of these specific details. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0035] Embodiments of the present invention provide a fibrous hydrogel-based multi-cell co-culture model, which includes multilayer aligned nanofiber membranes and GelMA hydrogel.

[0036] In a preferred embodiment, the multilayer oriented nanofiber membrane is a multilayer orthogonally oriented structure in which the fibers of a single layer are aligned and two adjacent layers are arranged orth...

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Abstract

The invention discloses a multi-cell co-culture model based on fiber hydrogel and a preparation method of the model. The multi-cell co-culture model comprises a multi-layer oriented nanofiber membraneand GelMA hydrogel which is poured into the multi-layer oriented nanofiber membrane to form gel and is of a three-dimensional porous structure. The multi-layer oriented nanofiber membrane can be prepared by utilizing an electrostatic spinning technology. By using the multi-cell co-culture model, a cell is inoculated on the multi-layer directional nanofiber membrane, a second cell is inoculated onthe upper surface of a fiber hydrogel stent, and a third cell is inoculated below the fiber hydrogel stent, so that in-vitro co-culture of various cells is realized. The multi-cell co-culture model not only can realize co-culture of multiple cells, but also has good mechanical properties and high bionic properties, and can provide structures and properties of extracellular matrixes capable of simulating natural tissues for the multiple cultured cells.

Description

technical field [0001] The invention relates to a multi-cell co-culture model, in particular to a fiber hydrogel-based multi-cell co-culture model and a preparation method thereof. Background technique [0002] Many tissues and organs in the human body are composed of a variety of cells. Different cells communicate with each other, influence each other, promote each other's biological behaviors such as proliferation and differentiation, and jointly maintain the homeostasis of tissues or organs. Therefore, constructing a multi-cell co-culture model in vitro can not only be a good bionic simulation, but also have better functionality. However, the traditional tissue engineering scaffolds can only achieve the culture of a single cell, which is far from enough to realize the in vitro biomimetic and simulation of the whole tissue or organ. Contents of the invention [0003] The purpose of the present invention is to provide a multi-cell co-culture model based on fiber hydrogel...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/38A61L27/18A61L27/22A61L27/50A61L27/52A61L27/56C12N5/071D01F6/84D04H1/728D04H1/435
CPCA61L27/18A61L27/222A61L27/38A61L27/50A61L27/52A61L27/56A61L2400/12A61L2430/16C12N5/0697C12N2533/30C12N2533/40C12N2533/50C12N2533/54D01F6/84D04H1/435D04H1/728C08L67/04
Inventor 杨思明弥胜利孔彬
Owner GENERAL HOSPITAL OF PLA
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