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Acridinium ester antibody labeling method and application thereof

An antibody labeling, acridine ester technology, applied in the field of biotechnology detection, can solve the problems of detection sensitivity, stability, and linear range that cannot meet clinical needs, and affect MPO, etc. The effect of marking method is simple

Inactive Publication Date: 2020-11-03
GOLDBIOMARKERS DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the detection methods of MPO are mostly enzyme-linked immunosorbent assay, turbidimetric method and colloidal gold method. Due to the limitation of methodology, the sensitivity, stability and linear range of the detection are difficult to meet the clinical needs, which affects the timely diagnosis and early treatment of MPO. play a role

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 (taking 50 tests / batch as example, the same below)

[0028] A method for labeling an acridinium ester antibody, the method comprising the following steps:

[0029] 1) Measure 3mL of 0.1mol / L, pH7.6 PB buffer solution into a centrifuge tube;

[0030] 2) Add 250 μg of myeloperoxidase detection antibody and mix well;

[0031] 3) Add 100 μL of acridinium ester solution (1 mg / mL), mix thoroughly, and shake at room temperature for 2 hours in the dark;

[0032] 4) Add 345 μL of 10% BSA solution, mix well, shake and react at room temperature for 30 minutes in the dark;

[0033] 5) Add an appropriate amount of labeling buffer to 6mL, and store in a sealed container at 2-8°C.

Embodiment 2

[0035] A method for labeling an acridinium ester antibody, the method comprising the following steps:

[0036] 1) Measure 3mL of 0.1mol / L, pH7.4 PB buffer solution in a centrifuge tube;

[0037] 2) Add 200 μg of myeloperoxidase detection antibody and mix well;

[0038] 3) Add 100 μL of acridinium ester solution (1 mg / mL), mix thoroughly, and shake at room temperature for 110 minutes in the dark;

[0039] 4) Add 345 μL of 10% BSA solution, shake and react at room temperature for 30 minutes in the dark;

[0040] 5) Add an appropriate amount of labeling buffer to 6mL, and store in a sealed container at 2-8°C.

Embodiment 3

[0042] A method for labeling an acridinium ester antibody, the method comprising the following steps:

[0043] 1) Measure 3mL of 0.1mol / L, pH7.6 PB buffer solution into a centrifuge tube;

[0044] 2) Add 200 μg of myeloperoxidase detection antibody and mix well;

[0045] 3) Add 100 μL of acridinium ester solution (1 mg / mL), mix thoroughly, and shake at room temperature for 130 minutes in the dark;

[0046] 4) Add 345 μL of 10% BSA solution, shake and react at room temperature for 25 minutes in the dark;

[0047] 5) Add an appropriate amount of labeling buffer to 6mL, and store in a sealed container at 2-8°C.

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PUM

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Abstract

The invention discloses an acridinium ester antibody labeling method and application thereof. The method comprises the following steps: 1) measuring a labeled buffer solution in a centrifuge tube; 2)adding a myeloperoxidase detection antibody, and fully and uniformly performing mixing; 3) adding an acridinium ester solution, fully and uniformly performing mixing, and carrying out shaking reactionat room temperature in a dark place; 4) adding a proper amount of confining liquid, and carrying out room-temperature lightproof oscillation reaction; and 5) adding a proper amount of a labeling buffer solution for quantification, and performing sealing and storing at 2-8 DEG C. According to the method, the MPO inspection product which is higher in sensitivity, wide in linear range and convenientto realize automation can be obtained.

Description

technical field [0001] The invention relates to the field of biotechnology detection, in particular to a method for labeling an acridinium ester antibody in the preparation process of a chemiluminescence detection kit. Background technique [0002] Cardiovascular disease is the first chronic disease in my country, and its death accounts for more than 40% of disease deaths, ranking first, with a disability rate of nearly 40%, which seriously threatens the life and health of individuals and brings huge economic burden and mental pain to families and society. . Moreover, in recent years, the younger trend of the disease has become more and more obvious, and sudden cardiac death in the 30s and 40s is not uncommon. Such a high rate of death and disability is mainly due to the sudden onset and rapid progress of the disease, and many patients cannot receive timely and effective treatment. Therefore, early detection and early treatment are powerful measures to avoid serious adverse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/53G01N33/531
CPCG01N21/763G01N33/53G01N33/531
Inventor 胡文波
Owner GOLDBIOMARKERS DIAGNOSTICS
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