Trapping method and cytotoxicity testing method for smoke aerosol of heating non-combustible cigarette

Abstract

The invention belongs to the technical field of tobacco toxicity analysis, and particularly relates to a trapping method and a cytotoxicity testing method for smoke aerosol of a heat-not-burn cigarette. The method for capturing the aerosol in the smoke of the heat-not-burn cigarette comprises the following steps: (1) in the process of smoking the heat-not-burn cigarette, capturing total particulate matters in the smoke by adopting a Cambridge filter, and absorbing smoke gas phase matters penetrating through the Cambridge filter by adopting dimethyl sulfoxide; and after the capture is finished,extracting the smoke total particulate matters captured on the Cambridge filter by adopting dimethyl sulfoxide, and forming a smoke aerosol capture object by the dimethyl sulfoxide with the smoke total particulate matters extracted and the dimethyl sulfoxide with the smoke gas-phase matters absorbed in the step (1). The invention establishes a novel smoke gas solvent trapping method for in-vitrotoxicity test of the heat-not-burn cigarette, achieves comprehensive trapping of smoke aerosol of the heat-not-burn cigarette, and provides a basis for comprehensive evaluation of toxicity test of theheat-not-burn cigarette.

Description

Technical field [0001] The present invention belongs to the technical field of tobacco toxicity analysis, in particular to a method of heating without combustion of the cigarette smoke aerosol trap and cytotoxicity testing methods. Background technique [0002] Heating the cigarette does not burn with the "heating rather than burning tobacco or tobacco sheet" characteristics, as compared with conventional cigarettes, occur in different ways aerosol fumes, without cracking the combustion process, reducing the harmful components of tobacco combustion temperature cleavage of the chemical composition of mainstream smoke emission is greatly reduced; compared with the electronic cigarette, tobacco feature can provide some experience for consumers. Based on the lower heating without burning harmful components of cigarette tobacco release characteristics and has the advantage of feeling, which is considered to be a promising new type of cigarette. Currently, in vitro toxic effects of cig...

AI Technical Summary

Problems solved by technology

[0005] In some existing technologies for trapping smoke gaseous phase substances used in cigarette smoke chemical analysis experiments, organic acids are used as gaseous phase substance leaching solutions, and lye such as sodium hydroxide is also used to adjust the pH value. These organic acids Alkaline and lye itself will cause damage to the cells. In addition, organic acids will react with some chemical components in the smoke, so that the chemical composition of the smoke will change during the subsequent toxicology test, which cannot truly reflect the actual smoke. Biological effects of ingredients

In some existing technologies for capturing gaseous phase substances in cigarette smoke cell experiments, the steps are relatively cumbersome, for example, the gaseous phase substances are captured by phosphate buffer solution (PBS) first, and then organic solvents are used for capture. Multi-stage capture method, and ice bath conditions are required when capturing gas phase components. In addition, PBS and acetone or ethyl acetate have different capture efficiencies for smoke components (different solvent properties), and the smoke captured by each solvent Gas components are also different, which will affect the test results of subsequent cell experiments, and there are large differences; some existing trapping solvents, such as acetone and ethyl acetate, have chemical characteristics such as flammability and toxicity, and have low boiling points. The 37°C ambient temperature of the culture condition has the potential impact of volatilization, resulting in large experimental errors; in addition, in the subsequent cytotoxicity test, the particle phase components and the gas phase components are tested separately, and all the smoke is not tested. And the test results of cytotoxicity obtained by different solvents are also different, and the test results are not comparable

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