Research method of pesticide-fertilizer composite seed coating agent for sorghum
A sorghum and medicinal fertilizer technology, applied in fertilizer mixture, seed coating/seed dressing, nitrogen fertilizer, etc., can solve the problem of less sorghum seed coating agent
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[0175] (2.1.2) Preparation of drug-containing medium
[0176] Dissolve the original powder of the drug with methanol + 0.1% TWEEN-80, and dilute it with sterile distilled water to make a 10g / L mother solution. Cool down to about 50°C after sterilizing, add 1mL of prepared medicines to 9mL medium and shake well to obtain medicine-containing medium containing medicines of various concentrations, add the same amount of sterile water as a control, each treatment Set up 3 repetitions;
[0177] (2.1.3) Toxicity determination
[0178] The mycelium growth rate method was used to measure. Put the pre-cultivated colonies of the tested strains on the ultra-clean workbench, use a puncher with a diameter of 5mm to punch out the bacteria disc along the same circumference of the edge of the colony, and then paste the side of the bacteria disc containing the hyphae on the side containing the drug. In the center of the culture medium, place it upside down in a constant temperature incubator...
Embodiment 1
[0280] The preparation of embodiment 1 suspension seed coating
[0281] 0.6% tebuconazole, 9% cyantraniliprole, 9% thiamethoxam, 1% borax, 0.5% zinc sulfate, 0.5% molybdate molybdate, 0.0026mg / L brassinolide, 1.5% sodium alginate , 3% NNO, 1% Tween 80, 1% Acid Scarlet and 72.9% water were put into an electromagnetic stirrer and mixed uniformly, and the mixture was placed in a high-speed emulsifying shearing machine to be sheared and emulsified to obtain a finished product.
Embodiment 2
[0282] The preparation method of embodiment 2 coated sorghum seeds
[0283] Place 500 g of selected sorghum seeds in a sterile dry wide-mouth conical flask, pour 10 mL of the suspension seed coating agent prepared in Example 1 into it, shake it by hand, shake it in a shaker for 10 minutes, and place Blow dry in a ventilated place.
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